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Determination of ADP-ribosyl arginine anomers by reverse-phase high-performance liquid chromatography
Analytical Biochemistry
|September 1, 1986
Abstract:
A simple and rapid method for the determination of ADP-ribosyl arginine anomers was devised. Analysis is performed by reverse-phase high-performance liquid chromatography on a 5-micron Cosmosil 5C18 column and uv detection. ADP-ribosylation of arginine by hen liver ADP-ribosyl-transferase and the effect of pH on anomerization are also presented.