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In clinical practice, the direct measurement of hepatic blood flow to evaluate liver function presents significant challenges due to the intricate and specialized nature of the necessary techniques. Consequently, healthcare professionals often rely on empirical estimates derived from thorough patient examinations and liver function tests to gauge liver health. Among the tools at their disposal, the Child–Pugh and MELD scoring systems stand out for their ability to categorize and assess...
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Exocrine glands are classified as unicellular and multicellular. The unicellular glands are scattered single cells, such as goblet cells, found in the mucous membranes of the small and large intestines. On the other hand, multicellular exocrine glands develop as secretory sheets, like the internal lining of the abdomen or chest. Such secretory sheets release their secretions directly into the lumen of these organs. In addition, some multicellular glands have deep-seated secretory units to...
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Fabrication of the Thermoplastic Microfluidic Channels
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A thermoplastic microfluidic microphysiological system to recapitulate hepatic function and multicellular

Shyam Sundhar Bale1, Andrea Manoppo1, Rebecca Thompson1

  • 1Draper, Cambridge, Massachusetts.

Biotechnology and Bioengineering
|April 10, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed a new microfluidic system using thermoplastic materials for advanced liver cell culture. This platform supports primary human hepatocytes, enabling better drug screening and disease modeling with enhanced cellular interactions.

Keywords:
Kupffer cellsLiver-on-chipco-culture modeldilution effectshepatocytesinflammationoxygen transportthermoplastics

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • In vitro models are crucial for drug screening and disease modeling.
  • Microphysiological systems (MPSs) offer advanced recapitulation of organ complexity.
  • Traditional MPSs using elastomers have limitations in structural rigidity for high-throughput applications.

Purpose of the Study:

  • To develop a novel microfluidic bilayer model using thermoplastic materials for hepatic cell culture.
  • To create a stable and functional platform for primary human hepatocytes (PHHs).
  • To enable high-throughput screening and disease modeling of liver functions and interactions.

Main Methods:

  • Fabrication of a microfluidic MPS with two overlapping channels separated by a porous membrane.
  • Culture of PHHs in a collagen sandwich gel format within the top channel.
  • Utilizing an oxygen-permeable material and media exchange via the bottom channel for PHH stabilization.
  • Co-culture of hepatocytes and Kupffer cells to study inflammatory responses.

Main Results:

  • Demonstrated successful PHH culture for 7 days with sustained stabilization, secretory, and metabolic functions.
  • Achieved a reduced media-to-cell ratio compared to traditional multi-well systems, enhancing sensitivity.
  • Captured cellular interactions and responses in a hepatocyte-Kupffer co-culture model under inflammatory stimulus.

Conclusions:

  • Thermoplastic materials offer advantages over elastomers for developing rigid, high-throughput microfluidic MPSs.
  • The developed microfluidic MPS platform supports stable primary human hepatocyte culture and co-culture.
  • This platform is a promising step towards advanced hepatic disease modeling and therapeutic response evaluation.