Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Binary Fission01:26

Binary Fission

2.6K
Binary fission is the primary mode of asexual reproduction in prokaryotes, such as bacteria. It results in the production of two genetically identical daughter cells. This highly efficient process ensures the rapid propagation of bacterial populations under favorable conditions and involves coordinated cellular and molecular events.DNA Replication and SeparationThe process begins with the replication of the bacterial chromosome. The circular DNA molecule unwinds at a specific origin of...
2.6K
Binary Fission01:20

Binary Fission

63.0K
Fission is the division of a single entity into two or more parts, which regenerate into separate entities that resemble the original. Organisms in the Archaea and Bacteria domains reproduce using binary fission, in which a parent cell splits into two parts that can each grow to the size of the original parent cell. This asexual method of reproduction produces cells that are all genetically identical.
63.0K
Quantitative Analysis01:12

Quantitative Analysis

1.4K
Quantitative analysis is a technique for measuring the amount of specific constituents in a sample. When the sample's composition is unknown, qualitative analysis is performed first to identify its components, which ensures that the correct substances are measured during the quantitative phase.
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the...
1.4K
Preparation and Reactions of Thiols02:33

Preparation and Reactions of Thiols

7.5K
Thiols are prepared using the hydrosulfide anion as a nucleophile in a nucleophilic substitution reaction with alkyl halides. For instance, bromobutane reacts with sodium hydrosulfide to give butanethiol.
7.5K
Structure and Nomenclature of Thiols and Sulfides02:17

Structure and Nomenclature of Thiols and Sulfides

5.7K
Thiols and sulfides are sulfur analogs of alcohols and ethers, respectively, where the sulfur atom takes the place of the oxygen atom. Thus, thiols are generally represented as RSH, where R is an alkyl substituent and —SH is the functional group. On the other hand, in sulfides, the central sulfur atom is bonded to two hydrocarbon groups on either side. Depending upon the type of group, sulfides can be either symmetrical or asymmetrical. Both thiols and sulfides display a bent geometry,...
5.7K
Scatter Plot01:15

Scatter Plot

10.8K
The most common and easiest way to display the relationship between two variables, x and y, is a scatter plot. A scatter plot shows the direction of a relationship between the variables. A clear direction happens when there is either:
10.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unraveling Multiregional Neural Patterns during Consciousness Transition Using Flexible Microelectrode Arrays Integrated with Neuropixels Chips.

Nano letters·2025
Same author

Upconversion Nanoparticle-Delivery Flexible Optrodes for Long-Lasting Multichannel Electrophysiology and Transcranial NIR Optogenetics.

ACS nano·2025
Same author

Spatially Precise Genetic Engineering at the Electrode-Tissue Interface.

Advanced materials (Deerfield Beach, Fla.)·2024
Same author

E-Tattoos: Toward Functional but Imperceptible Interfacing with Human Skin.

Chemical reviews·2024
Same author

Free-Standing Carbon Nanotube Embroidered Graphene Film Electrode Array for Stable Neural Interfacing.

Nano letters·2023
Same author

Ultraflexible Neural Probes for Multidirectional Neuronal Activity Recordings over Large Spatial and Temporal Scales.

Nano letters·2023

Related Experiment Video

Updated: Jan 26, 2026

Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy
10:59

Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy

Published on: May 12, 2023

3.5K

Binary Thiol-Capped Gold Nanoparticle Monolayer Films for Quantitative Surface-Enhanced Raman Scattering Analysis.

Huihui Tian1,2, Hongbian Li1,2, Ying Fang1,2,3

  • 1CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience , National Center for Nanoscience and Technology , Beijing 100190 , China.

ACS Applied Materials & Interfaces
|April 10, 2019
PubMed
Summary

Researchers developed a novel gold nanoparticle (AuNP) film for sensitive and quantitative surface-enhanced Raman scattering (SERS) analysis. This reproducible SERS substrate enables accurate detection of trace analytes, including fungicides in food.

Keywords:
gold nanoparticlemalachite greenmonolayer filmquantitative SERSself-assembly

More Related Videos

Observation and Analysis of Blinking Surface-enhanced Raman Scattering
05:52

Observation and Analysis of Blinking Surface-enhanced Raman Scattering

Published on: January 11, 2018

7.8K
Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

4.1K

Related Experiment Videos

Last Updated: Jan 26, 2026

Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy
10:59

Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy

Published on: May 12, 2023

3.5K
Observation and Analysis of Blinking Surface-enhanced Raman Scattering
05:52

Observation and Analysis of Blinking Surface-enhanced Raman Scattering

Published on: January 11, 2018

7.8K
Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

4.1K

Area of Science:

  • Nanotechnology
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Surface-enhanced Raman scattering (SERS) offers high sensitivity for molecular fingerprinting.
  • Reproducible SERS substrates with high-density hotspots are crucial for quantitative analysis but remain challenging.
  • Existing methods struggle with substrate consistency, limiting reliable quantitative SERS.

Purpose of the Study:

  • To design and fabricate a binary thiol-capped gold nanoparticle (AuNP) monolayer film (MLF) for sensitive and quantitative SERS.
  • To create a reproducible SERS substrate with controlled hotspots and integrated internal standards.
  • To demonstrate the substrate's capability for detecting trace analytes in complex matrices.

Main Methods:

  • Fabrication of AuNP MLF using binary thiol capping (dodecanethiol and 4-mercaptopyridine).
  • Controlled self-assembly of AuNPs to form ordered monolayers with optimized interparticle gaps.
  • Utilized 4-mercaptopyridine as an internal standard for quantitative SERS measurements.
  • Evaluated SERS performance using malachite green detection in aqueous solution and in situ detection in shrimp tissue.

Main Results:

  • Achieved a highly ordered MLF with high-density hotspots and uniformly distributed internal standards.
  • Demonstrated a high SERS enhancement factor of up to 3.3 × 107.
  • Obtained a low detection limit of 100 pM for malachite green.
  • Successfully performed in situ SERS detection of trace fungicide residues in shrimp tissue using the flexible and transparent substrate.

Conclusions:

  • The binary thiol-capped AuNP MLF is a reliable and reproducible SERS substrate for quantitative analysis.
  • The rational design enables precise control over hotspots and integration of internal standards.
  • The developed SERS substrate shows significant potential for sensitive and quantitative detection of trace contaminants in real-world samples.