Biostimulants application for bacterial metabolic activity promotion and sodium dodecyl sulfate degradation under...

In this study, the metabolic activity (adenosine triphosphate, ATP; electron transfer system, ETS; and dehydrogenase activity, DHA) response of a sodium dodecyl sulfate (SDS) degrading bacterium Pseudomonas sp. SDS-N2 to copper stress conditions were investigated. Results showed that the ATP content, ETS activity, and DHA activity of strain SDS-N2 were significantly correlated with substrate removal efficiency and bacterial growth under copper stress conditions. Based on the metabolic response patterns of strain SDS-N2, biostimulants citric acid, proline as well as FeSO₄ were used to promote the metabolic activity of strain SDS-N2 at 0.8 mg L^-1 copper stress condition. Plackett-Burman design and analysis proved that citric acid and FeSO₄ were significant factors for enhanced SDS removal; and the optimum biostimulation conditions (FeSO₄ 72 mg L^-1 and citric acid 100 mg L^-1) for SDS removal were obtained by using steepest ascent experiment and central composite design. Under the optimum biostimulation conditions, ATP, ETS, DHA activity as well as bacterial growth were 14.1, 45.5, 0.5 and 2.3-fold higher than that of the control (without FeSO₄ and citric acid addition) after 12.5 h biodegradation, and the substrate removal efficiency was increase by 37.6%.