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Bacillus subtilis rec Assay for Quantification of Aflatoxins
Miguel D'Aquino1, Silvia Bejar1, Ernesto Bollini1
1Department of Toxicology (Hygiene & Public Health), Faculty of Pharmacy and Biochemistry, Buenos Aires University, Junin 954, 1113 Buenos Aires, Argentina.
Abstract:
The Bacillus subtilis 1791 rec- assay was used to quantify genotoxic mycotoxins. This assay is based on detection of mycotoxin-produced DNA alterations arising from recombinational deficiency in rec- cells. Aflatoxin B1 showed a linear dose-response relationship when the inhibition halo was taken as a parameter for the evaluation procedure. Assays carried out with or without hepatic microsomal activation exhibited a similar response.
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