Related Experiment Video
Updated: Jan 26, 2026
10:57
NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
Published on: January 12, 2020
11.2K
Transfer Volume-Dependent Recovery of Salmonella from Minced Meat
1Agro-Technical Academy, Faculty of Veterinary Medicine, 10-957 Olsztyn-Kortowo, and Institute of Food and Nutrition, Powsinska 61/63, 02-903 Warsaw, Poland.
Journal of Food Protection
|April 11, 2019
Summary
This study investigated Salmonella recovery from frozen ground beef. Higher dilution rates improved Salmonella isolation purity, crucial for accurate food safety testing.
Area of Science:
- Food Microbiology
- Bacteriology
- Food Safety
Background:
- Salmonella contamination in ground beef poses a significant public health risk.
- Accurate detection methods are essential for ensuring food safety.
Purpose of the Study:
- To evaluate the impact of sample dilution on the recovery and purity of Salmonella from frozen ground beef.
- To determine optimal dilution factors for reliable Salmonella detection.
Main Methods:
- Ground beef samples were inoculated with Salmonella and stored frozen.
- Samples underwent pre-enrichment, serial 10-fold dilutions, and plating on selective media.
- Salmonella recovery rates and culture purity were assessed across different dilutions.
Main Results:
- Higher sample dilutions (10^-4 and beyond) yielded purer Salmonella cultures.
- Undiluted samples frequently contained overgrowing concomitant bacteria, hindering accurate enumeration.
- Recovery percentages varied by Salmonella serotype and dilution factor.
Conclusions:
- Serial dilution is critical for obtaining pure Salmonella cultures from ground beef.
- Optimizing dilution strategies enhances the reliability of Salmonella detection in food matrices.
- This research informs improved food safety testing protocols for Salmonella.
More Related Videos
12:50Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
Published on: June 9, 2014
15.0K
09:10Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
Published on: October 18, 2010
13.5K
Related Concept Videos
02:41Constant Volume Calorimetry
30.6K
Calorimeters are useful to determine the heat released or absorbed by a chemical reaction. Coffee cup calorimeters are designed to operate at constant (atmospheric) pressure and are convenient to measure heat flow (or enthalpy change) accompanying processes that occur in solution at constant pressure. A different type of calorimeter that operates at constant volume, colloquially known as a bomb calorimeter, is used to measure the energy produced by reactions that yield large amounts of heat and...
30.6K
01:21Frequency-dependent Selection
23.4K
When the fitness of a trait is influenced by how common it is (i.e., its frequency) relative to different traits within a population, this is referred to as frequency-dependent selection. Frequency-dependent selection may occur between species or within a single species. This type of selection can either be positive—with more common phenotypes having higher fitness—or negative, with rarer phenotypes conferring increased fitness.
23.4K
01:20Volume of Distribution
1.2K
The apparent volume of distribution (Vd) is a crucial pharmacokinetic parameter representing the hypothetical body fluid volume into which a drug disperses. It is calculated based on the total amount of drug in the body (estimated from the administered dose and bioavailability) divided by the plasma drug concentration. The total amount of drug in the body does not directly refer to the dose given but is derived by accounting for absorption, distribution, metabolism, and excretion processes.
1.2K
01:17Work Done During Volume Change
5.1K
In mechanics, work is done on an object when the force acting on it displaces the object. In thermodynamics, work done on a system can be estimated when the system's volume changes during any thermodynamic process.
Consider a gas confined to a cylinder fitted with a movable piston at one end. If the gas expands from volume V1 to volume V2, it exerts a force on the piston, such that the piston moves by a distance dr.
The work done by the gas on the piston can be expressed as
Consider a gas confined to a cylinder fitted with a movable piston at one end. If the gas expands from volume V1 to volume V2, it exerts a force on the piston, such that the piston moves by a distance dr.
The work done by the gas on the piston can be expressed as
5.1K
01:15Respiratory Volumes
2.9K
Respiratory volumes are crucial metrics, meticulously measured to quantify the air exchanged in and out of the lungs during various phases of the breathing cycle. These precise measurements are vital for assessing lung function, diagnosing respiratory conditions, and monitoring overall respiratory health. Each parameter provides specific insights into the mechanics of breathing and the functional capacity of the lungs.
Tidal Volume (TV) Tidal volume (TV) is the air inhaled or exhaled in a...
Tidal Volume (TV) Tidal volume (TV) is the air inhaled or exhaled in a...
2.9K
01:24Muscle Recovery and Fatigue
4.1K
Muscle fatigue refers to the decline in a muscle's ability to maintain the force of contraction after prolonged activity. It primarily stems from changes within muscle fibers. Even before experiencing muscle fatigue, one may feel tired and have the urge to stop the activity. This response, known as central fatigue, occurs due to changes in the central nervous system, namely the brain and spinal cord. While there is no single mechanism that induces fatigue, it may serve as a protective...
4.1K