Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

11.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.9K
Classification of Titrimetric Analysis Based on Reaction Types01:01

Classification of Titrimetric Analysis Based on Reaction Types

1.5K
Titrimetric analysis in solution chemistry involves measuring the volume of solutions and is often called volumetric analysis. The standard solution of known concentration in the burette is called the titrant, whereas the solution of unknown concentration in the flask is called the analyte, or titrand. Titrimetric analyses can be classified into four types based on the reactions between the titrant and analyte.
Titrations between an acid and a base lead to neutralization reactions that form...
1.5K
Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

14.7K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
14.7K
Dimensional Analysis03:40

Dimensional Analysis

60.9K
Dimensional analysis, also known as the factor label method, is a versatile approach for mathematical operations. The main principle behind this approach is: the units of quantities must be subjected to the same mathematical operations as their associated numbers. This method can be applied to computations ranging from simple unit conversions to more complex and multi-step calculations involving several different quantities and their units.
Conversion Factors and Dimensional Analysis
The unit...
60.9K
DNA Base Pairing02:27

DNA Base Pairing

33.1K
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
33.1K
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

7.9K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and validation of an artificial intelligence classifier for histologic grading of superficial non-ampullary duodenal epithelial tumors.

Endoscopy international open·2026
Same author

Correction: Ishida et al. Wnt/β-Catenin Signaling Regulates Hepatitis B Virus cccDNA Levels. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 6942.

International journal of molecular sciences·2026
Same author

Safety and Efficacy of Oral Tacrolimus Induction Therapy in Elderly Patients with Ulcerative Colitis: A Subanalysis of a Large Multicenter Retrospective Cohort Study.

Gerontology·2026
Same author

Hypoattenuation Pattern on Contrast-Enhanced Computed Tomography Predicts Poor Prognosis in Patients with Pancreatic Neuroendocrine Tumors.

Journal of clinical medicine·2026
Same author

Elucidation of the process of delayed colonic perforation after endoscopic thermal injury in a rat model.

Scientific reports·2026
Same author

Single-Cell Analysis Identified a Recurrent Malignant-Associated Transcriptional State in Colorectal Cancer.

Cancer science·2026

Related Experiment Video

Updated: Jan 26, 2026

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
06:24

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

4.1K

Nx1-Seq (Well Based Single-Cell Analysis System).

Shinichi Hashimoto1

  • 1Kanazawa University, Kanazawa, Ishikawa, Japan. hashimoto@med.kanazawa-u.ac.jp.

Advances in Experimental Medicine and Biology
|April 11, 2019
PubMed
Summary
This summary is machine-generated.

Single-cell gene expression analysis reveals tissue complexity and cellular diversity. This study introduces a novel well-based single-cell transcriptome method for enhanced cellular analysis.

Keywords:
Barcoded beadsCancerEmulsion PCRMicrowellMolecular barcodingNext-generation sequencingSingle cellsT cell receptorTranscriptome

More Related Videos

Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies
05:45

Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies

Published on: March 29, 2024

3.4K
Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
06:22

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

Published on: August 25, 2020

13.4K

Related Experiment Videos

Last Updated: Jan 26, 2026

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
06:24

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

4.1K
Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies
05:45

Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies

Published on: March 29, 2024

3.4K
Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
06:22

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

Published on: August 25, 2020

13.4K

Area of Science:

  • Molecular Biology
  • Genomics
  • Cell Biology

Background:

  • Traditional methods struggle to analyze continuous cell differentiation and identify cells with unclear markers.
  • Cell populations previously considered homogeneous exhibit significant diversity.
  • Understanding tissue microenvironments and intercellular interactions is crucial for disease research.

Purpose of the Study:

  • To address limitations in analyzing cell differentiation and heterogeneity.
  • To introduce a novel, well-based single-cell transcriptome method.
  • To enhance the study of intercellular interactions and identify specific marker genes.

Main Methods:

  • Development of a new well-based single-cell transcriptome profiling technique.
  • Integration of existing methods for comprehensive single-cell analysis.
  • Analysis of hundreds of single cells to capture population diversity.

Main Results:

  • The new method facilitates detailed analysis of individual cell populations.
  • Single-cell analysis provides insights into tissue microenvironment complexity.
  • The approach aids in identifying specific marker genes and understanding cellular diversity.

Conclusions:

  • The developed well-based single-cell transcriptome method offers a powerful tool for biological research.
  • Single-cell analysis is essential for understanding complex biological systems and diseases.
  • This technique advances the study of cell differentiation and heterogeneity.