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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
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Tumor Progression

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Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
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Related Experiment Video

Updated: May 5, 2026

In vitro Organoid Culture of Primary Mouse Colon Tumors
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Single-Cell Analysis Identified a Recurrent Malignant-Associated Transcriptional State in Colorectal Cancer.

Sadahiro Iwabuchi1,2, Kentaro Sawada3,4, Motohiro Kojima5,6

  • 1Department of Molecular Pathophysiology, Institute of Advanced Medicine, Wakayama Medical University, Wakayama-shi, Wakayama, Japan.

Cancer Science
|March 2, 2026
PubMed
Summary

Single-cell sequencing of Japanese colorectal cancer (CRC) revealed a distinct malignant cell cluster. This cluster showed high KCNQ1OT1 and low RASSF6 expression, offering new insights into CRC molecular subtypes.

Keywords:
FOXQ1KCNQ1OT1RASSF6colorectal cancersingle cell RNA sequencing

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Area of Science:

  • Genomics
  • Oncology
  • Molecular Biology

Background:

  • Colorectal cancer (CRC) displays significant molecular heterogeneity.
  • Limited single-cell transcriptomic data exists for Japanese CRC patients.

Purpose of the Study:

  • To characterize malignant cell populations in Japanese CRC using single-cell RNA sequencing (scRNA-seq).
  • To identify novel molecular subtypes and gene expression patterns in CRC.

Main Methods:

  • Performed scRNA-seq on tumor and adjacent normal tissues from four Japanese CRC patients.
  • Analyzed gene expression patterns, focusing on long noncoding RNAs and tumor suppressors.
  • Reanalyzed public bulk RNA-seq data from East Asian cohorts.

Main Results:

  • Identified a common cancer-associated cell cluster across all patients, marked by high KCNQ1OT1 expression.
  • Observed a significant decrease in Ras Association Domain Family Member 6 (RASSF6) within this cluster.
  • Confirmed reduced RASSF6 expression in tumor tissues from independent East Asian cohorts via bulk RNA-seq.

Conclusions:

  • Single-cell analysis revealed a recurrent cancer-associated transcriptional state in Japanese CRC.
  • Distinct gene expression features, like altered RASSF6 and KCNQ1OT1 levels, may be missed by bulk analyses.
  • Highlights the importance of single-cell profiling for refining molecular understanding and potential therapeutic targets in CRC.