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Related Concept Videos

What is Variation?01:14

What is Variation?

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Apart from the measures of central tendency, distribution, outliers, and the changing characteristics of data with time, an important characteristic of any data set is its variation or spread. In some data sets, the data values are concentrated closely near the mean; in others, the data values are more widely spread out from the mean.
The range, standard deviation, standard error, and variance are the different measures of variation.
Range: The range is the difference between its maximum and...
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Variation01:19

Variation

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An important characteristic of any set of data is the variation in the data. In some data sets, the data values are concentrated closely near the mean; in other data sets, the data values are more widely spread out from the mean. The most common measure of variation, or spread, is the standard deviation, which is the square root of variance.
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Plant Cells and Tissues02:01

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Plant tissues are collections of similar cells performing related functions. Different plant tissues will have their own specialized roles and can be combined with other tissues to form organs such as flowers, fruit, stem, and leaves. Two major types of plant tissue include meristematic and permanent tissue.
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Conservative Site-specific Recombination and Phase Variation02:53

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Epithelial Tissues and Their Functions01:23

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Epithelial tissues are large sheets of cells covering all of the surfaces of the body. These surfaces can be internal or external, for example, skin, airways, the digestive tract, the urinary system, and the reproductive system. Hollow organs and body cavities that do not connect to the body's exterior, including blood vessels and serous membranes, are lined by epithelial tissue known as the endothelium.
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Functions of Connective Tissues01:17

Functions of Connective Tissues

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Connective tissues perform a broad range of functions in the body. Their primary function is to connect and link different tissues in the body and act as packaging material between tissues. The areolar tissue, a connective tissue prototype, commonly cements various tissue types in diverse body organs. In contrast, adipose tissue cushions internal organs while insulating the body from heat loss.
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Updated: Jan 26, 2026

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy
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Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

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Phenotypic Variation Between Stromal Cells Differentially Impacts Engineered Cardiac Tissue Function.

Tracy A Hookway1, Oriane B Matthys1,2, Federico N Mendoza-Camacho1

  • 11 Gladstone Institute of Cardiovascular Disease, San Francisco, California.

Tissue Engineering. Part A
|April 11, 2019
PubMed
Summary
This summary is machine-generated.

Choosing the right supporting stromal cells is crucial for developing functional stem cell-derived cardiac tissues. Different stromal cell types significantly impact cardiac tissue development and function.

Keywords:
cardiac microtissuescardiomyocytesfibroblastsheterotypic interactionsstem cell-derived tissuesstromal cells

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Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Tissue Engineering

Background:

  • Recapitulating native tissue complexity requires understanding cell-cell interactions.
  • Stromal cells are essential for stable stem cell-derived cardiac tissue formation.
  • Previous studies used diverse stromal cell types, leading to variability.

Purpose of the Study:

  • To systematically characterize various stromal populations for cardiac tissue engineering.
  • To investigate the impact of stromal cell phenotype and morphology on cardiac tissue.
  • To determine how stromal choice affects induced pluripotent stem cell-cardiomyocyte (iPSC-CM) phenotype and function.

Main Methods:

  • Systematic characterization of multiple stromal cell populations.
  • Assessment of stromal cell phenotype and morphology.
  • Evaluation of cardiac tissue function and iPSC-CM phenotype in co-culture systems.

Main Results:

  • Significant variability observed in stromal cell phenotype and morphology based on cell source.
  • Differential impact of stromal populations on engineered cardiac tissue function.
  • Stromal cell choice influenced the phenotypic maturation of iPSC-CMs.

Conclusions:

  • The selection of supporting stromal populations critically influences engineered cardiac tissue performance.
  • Stromal cell characteristics must be carefully considered for optimizing cardiac tissue engineering outcomes.
  • Further research is needed to identify optimal stromal partners for specific cardiac applications.