Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Extracellular Matrix01:42

The Extracellular Matrix

88.1K
Overview
88.1K
The Extracellular Matrix01:29

The Extracellular Matrix

11.9K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
11.9K
Extracellular Matrix01:26

Extracellular Matrix

5.1K
Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
5.1K
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

3.4K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Short-Term Electrical Stimulation Impacts Cardiac Cell Structure and Function.

Journal of tissue engineering and regenerative medicine·2025
Same author

Extragenital self-collection testing for gonorrhea and chlamydia: A feasibility study for expanding STI screening in the Veterans Health Administration.

American journal of clinical pathology·2024
Same author

Exogenous ECM in an environmentally-mediated <i>in vitro</i> model for cardiac fibrosis.

bioRxiv : the preprint server for biology·2024
Same author

Digital phenotyping of depression during pregnancy using self-report data.

Journal of affective disorders·2024
Same author

The effects of brain serotonin deficiency on the behavioral and neurogenesis-promoting effects of voluntary exercise in tryptophan hydroxylase 2 (R439H) knock-in mice.

Neuropharmacology·2024
Same author

Engineered Cardiac Microtissue Biomanufacturing Using Human Induced Pluripotent Stem Cell Derived Epicardial Cells.

bioRxiv : the preprint server for biology·2024
Same journal

Development and Evaluation of Polyvinyl Alcohol/Carboxymethyl Chitosan Hydrocolloid Incorporating Cynodon dactylon Extract and Zinc Oxide Nanoparticle for Bedsore Healing in a Wistar Rat Model.

Journal of biomedical materials research. Part A·2026
Same journal

Engineering Bone-Targeted LNP Delivery of Anti-Sclerostin Antibody mRNA for the Treatment of Osteoporosis.

Journal of biomedical materials research. Part A·2026
Same journal

Substrate Stiffness and Viscoelasticity Influence Fibroblast Senescence.

Journal of biomedical materials research. Part A·2026
Same journal

Biomaterial-Focused Strategies Targeting Dendritic Cells for Autoimmune Disease Treatment.

Journal of biomedical materials research. Part A·2026
Same journal

Engineering Pro-Osteogenic Poly(l-Lactide-co-ε-caprolactone) Sponges Through Carbonate Apatite Integration.

Journal of biomedical materials research. Part A·2026
Same journal

Evaluation of Ethyl Cellulose-Ethanol Ablation in a Human-Sized 3D Construct of High-Grade Cervical Dysplasia.

Journal of biomedical materials research. Part A·2026
See all related articles

Related Experiment Video

Updated: Jan 14, 2026

Author Spotlight: Studying Cardiac Cell-Matrix Interactions In Vitro
06:47

Author Spotlight: Studying Cardiac Cell-Matrix Interactions In Vitro

Published on: March 22, 2024

2.0K

Exogenous Extracellular Matrix in an Environmentally-Mediated In Vitro Model for Cardiac Fibrosis.

Natalie Pachter1, Kristen Allen1, Tracy A Hookway1

  • 1Department of Biomedical Engineering, Binghamton University, New York, New York, USA.

Journal of Biomedical Materials Research. Part A
|October 23, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a tunable in vitro cardiac fibrosis model using cardiomyocytes, fibroblasts, and extracellular matrix (ECM) proteins. This model aids in understanding fibrotic diseases and screening potential therapeutics.

Keywords:
cardiac fibroblastscardiac fibrosiscardiac tissue engineeringcardiomyocytescollagenextracellular matrixinduced pluripotent stem cells

More Related Videos

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix
10:21

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Published on: June 14, 2016

10.6K
Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
07:21

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

Published on: December 26, 2019

8.0K

Related Experiment Videos

Last Updated: Jan 14, 2026

Author Spotlight: Studying Cardiac Cell-Matrix Interactions In Vitro
06:47

Author Spotlight: Studying Cardiac Cell-Matrix Interactions In Vitro

Published on: March 22, 2024

2.0K
Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix
10:21

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Published on: June 14, 2016

10.6K
Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
07:21

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

Published on: December 26, 2019

8.0K

Area of Science:

  • Biomedical Engineering
  • Cardiovascular Research
  • Tissue Engineering

Background:

  • Cardiac fibrosis, a key factor in heart disease, lacks effective clinical treatments.
  • Understanding fibrotic mechanisms and screening therapeutics requires advanced in vitro models.

Purpose of the Study:

  • To develop a tunable in vitro cardiac fibrosis model.
  • To investigate the impact of extracellular matrix (ECM) modulation on cardiac tissue formation and function.
  • To establish a platform for therapeutic screening.

Main Methods:

  • Combined cardiomyocytes, cardiac fibroblasts, and exogenous ECM proteins (collagen I).
  • Created two cardiac tissue constructs: aggregates and tissue rings.
  • Assessed tissue formation, cellular distribution, and contractile function.
  • Utilized histological analysis to confirm ECM incorporation.

Main Results:

  • Collagen I addition significantly impacted aggregate formation but had less effect on ring formation.
  • Modulated ECM did not severely impair contractile function in either tissue type.
  • Histological analysis confirmed direct incorporation of exogenous ECM into tissues.
  • ECM modulation influenced tissue formation and cell distribution.

Conclusions:

  • The developed model allows for tunable modulation of the cardiac cell microenvironment.
  • This in vitro system is a valuable platform for studying cellular responses to ECM changes.
  • The model shows potential for screening therapeutic compounds for cardiac fibrosis.