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Deltamethrin-Induced Immunotoxicity and its Protection by Quercetin: An Experimental Study
Anoop Kumar1,2, Meenakshi Gupta3, Ruchika Sharma4
1Department of Pharmaceutical Science and Technology, Birla Institute of Technology, Mesra, Ranchi -835215, Jharkhand, India.
Background:
Deltamethrin (DLM) is a type 2 pyrethroid insecticide used in agriculture and home to control pests. However, emerging reports have indicated the immunotoxicity of DLM.
Objective:
Thus, in the current investigation, we have checked the immune-protective role of quercetin in DLM-induced immunotoxicity by using in silico and in vitro techniques.
Results:
In silico results have shown good interaction of quercetin towards immune cell receptors (T & B cell receptors). The findings of in vitro studies indicated the decrease in oxidative stress which is elevated by DLM in concentration & time-dependent manner. The increased caspases-3 activity was decreased by treatment of quercetin. The apoptosis induced by DLM in thymus and spleen was suppressed only at higher concentration (50μg/ml) of quercetin. Finally, the phenotypic changes due to DLM were restored by quercetin.
Conclusion:
Quercetin has strong binding affinity towards CD4, CD8 and CD28, CD45 receptors and protects the thymocytes and splenocytes against DLM-induced apoptotic signaling pathways.
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