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High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis QDB
Published on: August 21, 2018
Quantitative Dot Blot (QDB) as a universal platform for absolute quantification of tissue biomarkers
Wenfeng Zhang1, Guohua Yu2, Yunyun Zhang1
1Yantai Quanticision Diagnostics Inc., A Division of Quanticision Diagnostics, Inc. (US), Yantai Hi-tech District, Shandong, 264670, PR China.
A new Quantitative Dot Blot (QDB) method simplifies tissue biomarker analysis. This method accurately quantifies breast cancer biomarkers like Estrogen Receptor (ER) and Ki67, offering an efficient alternative to ELISA.
Area of Science:
- Biochemistry
- Oncology
- Biotechnology
Background:
- Sandwich ELISA methods for tissue biomarker quantitation face limitations.
- Developing and validating immunoassays can be resource-intensive.
Purpose of the Study:
- To introduce and validate the Quantitative Dot Blot (QDB) method as an alternative to Sandwich ELISA.
- To assess QDB's efficiency for absolute quantitation of key breast cancer biomarkers.
Main Methods:
- Developed QDB method using clinically validated immunohistochemistry (IHC) antibodies.
- Absolute quantitation of Estrogen Receptor (ER), Progesterone Receptor (PR), Ki67, and Her2 protein levels.
- Analyzed 190 frozen breast tissue biopsies.
Main Results:
- Successfully performed absolute quantitation of ER, PR, Ki67, and Her2.
- QDB results were validated against existing IHC data.
- Demonstrated QDB's capability for precise biomarker measurement.
Conclusions:
- QDB method offers a viable alternative to Sandwich ELISA for absolute tissue biomarker quantitation.
- QDB significantly reduces assay development effort and time.
- This method is suitable for analyzing biomarkers such as ER, PR, Ki67, and Her2 in breast cancer tissues.
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