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Updated: Jan 26, 2026

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
Published on: July 23, 2014
Polymerization of Various Lignins via Immobilized Myceliophthora thermophila Laccase (MtL)
Daniela Huber1, Alessandro Pellis2, Andreas Daxbacher3
1Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences, Vienna, Konrad Lorenz Str. 20, 3430 Tulln, Austria. daniela.huber@boku.ac.at.
Abstract:
Enzymatic polymerization of lignin is an environmentally-friendly and sustainable method that is investigated for its potential in opening-up new applications of one of the most abundant biopolymers on our planet. In this work, the laccase from Myceliophthora thermophila was successfully immobilized onto Accurel MP1000 beads (67% of protein bound to the polymeric carrier) and the biocatalyzed oxidation of Kraft lignin (KL) and lignosulfonate (LS) were carried out. Fluorescence intensity determination, phenol content analysis and size exclusion chromatography were performed in order to elucidate the extent of the polymerization reaction. The collected results show an 8.5-fold decrease of the LS samples' fluorescence intensity after laccase-mediated oxidation and a 12-fold increase of the weight average molecular weight was obtained.
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