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Author Spotlight: Advancing Tear Fluid Analysis Using a Standardized Protocol for Proteomics Research
Published on: December 1, 2023
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Age-associated changes in human tear proteome
Janika Nättinen1, Antti Jylhä1, Ulla Aapola1
11SILK, Department of Ophthalmology, Faculty of Medicine and Health Technology, Tampere University, PL 100, 33014 Tampere, Finland.
Clinical Proteomics
|April 13, 2019
Summary
Aging significantly alters tear fluid protein profiles, increasing inflammation and immune responses while decreasing cell growth. These findings reveal age-related changes in the ocular surface proteome, mirroring patterns seen in other tissues.
Area of Science:
- Ophthalmology
- Proteomics
- Gerontology
Background:
- Ocular surface diseases increase with age.
- Aging effects on tear film and ocular surface are under-researched.
- Normal aging impacts tear film composition.
Purpose of the Study:
- Investigate age-related changes in tear fluid proteomics.
- Identify proteins and biological functions affected by aging in the tear film.
- Understand the impact of aging on ocular surface health.
Main Methods:
- Collected tear fluid samples from 115 subjects undergoing eye surgery.
- Analyzed samples using NanoLC-TripleTOF mass spectrometry.
- Quantified 849 proteins in non-pooled tear samples.
Main Results:
- 17 tear proteins showed significant age-related correlation.
- Proteins linked to inflammation, immune response, and cell death increased with age.
- Cell growth and survival decreased, while immune response and inflammation increased.
Conclusions:
- Tear fluid protein profiles exhibit common age-dependent alterations.
- Aging affects biological functions in the tear film similarly to other tissues.
- Identified age-associated changes in ocular surface proteome.
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