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Emergence of plasmid-encoded VIM-2-producing Pseudomonas aeruginosa isolated from clinical samples in Lebanon

T Nawfal Dagher1,2, C Al-Bayssari3, S M Diene1

  • 1Aix Marseille Univ, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Faculté de Médecine et de Pharmacie, 19-21 boulevard Jean Moulin, 13385 Marseille, Cedex 05 France.

New Microbes and New Infections
|April 13, 2019
PubMed

Insights

Carbapenem-resistant Pseudomonas aeruginosa, a growing threat, has emerged in Lebanon. This study identifies the blaVIM-2 gene and oprD mutations as key drivers of this resistance in clinical isolates.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Genetics

Background:

  • Pseudomonas aeruginosa is an opportunistic pathogen.
  • Carbapenem resistance is a significant global health concern.
  • Limited data exists on carbapenem-resistant P. aeruginosa in Lebanon.

Purpose of the Study:

  • To describe the emergence of carbapenem-resistant Pseudomonas aeruginosa in Lebanese clinical settings.
  • To identify the genetic mechanisms conferring carbapenem resistance.
  • To report the first description of the blaVIM-2 gene in Lebanon.

Main Methods:

  • Bacterial isolation and identification.
  • Antimicrobial susceptibility testing.
  • Molecular detection of carbapenemase genes (blaVIM-2).
  • Analysis of oprD gene mutations.

Main Results:

  • Isolation of carbapenem-resistant Pseudomonas aeruginosa from Lebanese patients.
  • Detection of the plasmid-encoded blaVIM-2 gene in resistant isolates.
  • Identification of mutations disrupting the oprD gene.

Conclusions:

  • The blaVIM-2 gene is a primary mechanism of carbapenem resistance in P. aeruginosa in Lebanon.
  • OprD gene disruption contributes to carbapenem resistance.
  • This emergence necessitates enhanced surveillance and infection control measures.
Keywords:
Carbapenem resistancePseudomonas aeruginosaVIM-2oprDgene

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