Recent advances in open tubular capillary liquid chromatography
Shing Chung Lam1, Estrella Sanz Rodriguez1, Paul R Haddad1
1ASTech, ARC Training Centre for Portable Analytical Separation Technologies (ASTech), and Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences, University of Tasmania, Private Bag 75, Hobart, Tasmania 7001, Australia. Brett.Paull@utas.edu.au.
The Analyst
|April 13, 2019
Summary
Open tubular capillary liquid chromatography (OT-LC) offers superior efficiency and peak capacity. This review highlights advances in stationary phase development, column formats, and detection for wider applications beyond proteomics.
Area of Science:
- Analytical Chemistry
- Chromatography
Background:
- Open tubular capillary liquid chromatography (OT-LC) presents potential advantages over packed and monolithic columns.
- These benefits include enhanced column efficiency, peak capacity, and permeability.
- However, widespread application beyond proteomics is limited.
Purpose of the Study:
- To review advances and applications of OT-LC between 2007-2018.
- To focus on diverse separation mechanisms and applications enabled by modified capillary inner walls.
- To highlight developments in stationary phases, column formats, and detection.
Main Methods:
- Review of literature on open tubular capillary liquid chromatography.
- Analysis of advancements in stationary phase modification and formation.
- Examination of new column dimensions and formats.
- Discussion of detection techniques for OT-LC.
Main Results:
- Significant progress in stationary phase development and formation for OT-LC.
- Emergence of new column formats and dimensions tailored for specific applications.
- Improvements in detection methods enhancing OT-LC capabilities.
- Demonstrated potential for wider applications beyond proteomics.
Conclusions:
- OT-LC, with modified stationary phases, offers diverse separation mechanisms and applications.
- Advances in column technology and detection expand its utility.
- OT-LC holds significant future potential for various analytical challenges.
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