Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromosome Replication02:31

Chromosome Replication

10.5K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
10.5K
The DNA Replication Fork01:02

The DNA Replication Fork

40.7K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
40.7K
DNA Replication02:40

DNA Replication

59.0K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
59.0K
Replication in Prokaryotes02:35

Replication in Prokaryotes

97.6K
Overview
97.6K
Replication in Prokaryotes01:32

Replication in Prokaryotes

27.7K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
27.7K
Replication in Eukaryotes02:31

Replication in Eukaryotes

204.3K
Overview
204.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Platelet parameters and platelet Toll-like receptor 4 (TLR4) expression in patients with sepsis, and the effect of a joint treatment-plan integrating traditional Chinese and western medicine: a clinical study].

Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue·2011
Same author

A novel kernel Fisher discriminant analysis: constructing informative kernel by decision tree ensemble for metabolomics data analysis.

Analytica chimica acta·2011
Same author

Anterior debridement and reconstruction via thoracoscopy-assisted mini-open approach for the treatment of thoracic spinal tuberculosis: minimum 5-year follow-up.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2011
Same author

[A family-based association study of FXYD6 gene polymorphisms and schizophrenia].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics·2011
Same author

Prenatal diagnosis of penoscrotal transposition with 2- and 3-dimensional ultrasonography.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine·2011
Same author

Differentiation of α- or β-aspartic isomers in the heptapeptides by the fragments of [M + Na]+ using ion trap tandem mass spectrometry.

Journal of the American Society for Mass Spectrometry·2011

Related Experiment Video

Updated: Jan 26, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.2K

Mammalian CST averts replication failure by preventing G-quadruplex accumulation.

Miaomiao Zhang1, Bing Wang1, Tingfang Li1

  • 1Department of Genetics, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, PR China.

Nucleic Acids Research
|April 13, 2019
PubMed
Summary

The human CST complex resolves replication issues by unfolding G-quadruplex (G4) DNA. This prevents telomere loss and maintains genome integrity, particularly impacting lagging strand synthesis.

More Related Videos

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay
10:32

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay

Published on: February 3, 2022

7.8K
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.6K

Related Experiment Videos

Last Updated: Jan 26, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.2K
Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay
10:32

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay

Published on: February 3, 2022

7.8K
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.6K

Area of Science:

  • Molecular Biology
  • Genetics
  • DNA Replication

Background:

  • The human CST (CTC1-STN1-TEN1) complex interacts with G-rich single-strand DNA.
  • CST is known to help resolve replication problems at telomeres and genome-wide.
  • Previous in vitro studies showed CST disrupts G-quadruplex (G4) DNA structures.

Purpose of the Study:

  • To investigate the in vivo role of CST in resolving G-quadruplex (G4) DNA structures.
  • To determine CST's function in preventing replication blocks caused by G4s.
  • To elucidate CST's contribution to maintaining genome and telomere integrity.

Main Methods:

  • Assessed CST's G4 binding and unfolding efficiency compared to RPA.
  • Observed CST recruitment to chromatin upon G4 stabilization in cells, even with ATR/ATM inhibition.
  • Utilized STN1 depletion and G4 stabilization to study telomere replication, employing multi-telomere FISH and BrdU incorporation assays.

Main Results:

  • CST binds and unfolds G4 DNA with efficiency comparable to RPA.
  • CST is recruited to telomeric and non-telomeric chromatin when G4s are stabilized.
  • STN1 depletion leads to increased G4 accumulation, slowed DNA replication, and telomere loss, specifically affecting C-strand and lagging strand synthesis.

Conclusions:

  • CST plays a crucial role in resolving G4 structures, preventing replication fork stalling.
  • CST is essential for maintaining telomere duplex replication and overall genome integrity.
  • Findings reveal a novel function for CST in resolving G4s ahead of the fork and on the lagging strand template.