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Updated: Jan 26, 2026

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells
Published on: May 22, 2014
Generation of Neural Crest Progenitors from Human Pluripotent Stem Cells
Kwaku Dad Abu-Bonsrah1,2, Serena Viventi3, Donald F Newgreen2
1Department of Paedriatrics, University of Melbourne, Parkville, VIC, Australia.
Abstract:
There are a vast range of diseases and disorders that are neurocristopathic in origin, including Hirschsprung's disease, pheochromocytoma, familial dysautonomia, craniofacial disorders, and melanomas. Having a source of human neural crest cells is highly valuable for investigating potential treatments for such diseases. This chapter describes a robust and well-characterized protocol for deriving neural crest from human pluripotent stem cells (hPSCs), which can then be differentiated to neuronal and non-neuronal lineages. The protocol is adapted to suit hPSC maintenance as a monolayer bulk culture or as manual-passaged colonies, which makes it widely applicable to researchers that may use different systems for hPSC maintenance.
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