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Effect of Sodium Chloride on Uptake of Substrate by Staphylococcus aureus 196E

J L Smith1, M J Maurer1, M M Bencivengo1

  • 1Eastern Regional Research Center, U.S. Department of Agriculture, 600 East Mermaid Lane, Philadelphia, Pennsylvania 19118.

Journal of Food Protection
|April 14, 2019
PubMed

Insights

Sodium chloride inhibits Staphylococcus aureus growth and biochemical processes. This salt impacts substrate transport into cells, affecting key functions like enzyme activity and glucose utilization.

Area of Science:

  • Microbiology
  • Biochemistry
  • Cell Biology

Background:

  • Staphylococcus aureus is a common pathogen.
  • Understanding how external factors like sodium chloride affect bacterial physiology is crucial for developing control strategies.

Purpose of the Study:

  • To investigate the inhibitory effects of sodium chloride (NaCl) on various biochemical parameters in Staphylococcus aureus 196E.
  • To determine the specificity of NaCl's inhibitory action and explore potential mechanisms.

Main Methods:

  • Assessing the impact of NaCl on bacterial growth, enzyme induction (phospho-β-galactosidase), enterotoxin A synthesis, glucose utilization, substrate uptake (¹⁴C-2-deoxyglucose), and respiratory activity.
  • Evaluating the effect of various solutes and ion modulators (ionophores, ATPase inhibitors, ion channel blockers) on NaCl-induced inhibition.

Main Results:

  • NaCl significantly inhibited multiple biochemical parameters, including enzyme activity and glucose utilization, more than growth.
  • The inhibitory effect of NaCl on o-nitrophenyl-β-galactoside (ONPG) breakdown was reversed by ion modulators, suggesting a role for ion transport.
  • However, these modulators did not reverse NaCl's inhibition of glucose utilization, indicating a complex mechanism.

Conclusions:

  • Sodium chloride, and likely other solutes, exerts a general inhibitory effect on substrate transport into Staphylococcus aureus cells.
  • The findings suggest that NaCl interferes with the transport mechanisms essential for bacterial metabolism and function.

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