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Related Concept Videos

Electron Carriers01:24

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Electron carriers can be thought of as electron shuttles. These compounds can easily accept electrons (i.e., be reduced) or lose them (i.e., be oxidized). They play an essential role in energy production because cellular respiration is contingent on the flow of electrons.
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Updated: Jan 26, 2026

Carrier Lifetime Measurements in Semiconductors through the Microwave Photoconductivity Decay Method
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pH-Triggered Sheddable Shielding System for Polycationic Gene Carriers.

Jialiang Xia1,2, Huayu Tian3, Jie Chen4

  • 1School of Chemistry and Chemical Engineering, Lingnan Normal University, 29 Cunjin Road, Zhanjiang 524048, China. xiajl105@126.com.

Polymers
|April 14, 2019
PubMed
Summary

This study developed pH-sensitive PEG-b-PLL-g-succinylsulfathiazole (PPSD) to shield DNA/PEI polyplexes for gene delivery. PPSD shows potential for pH-triggered shielding and release, improving in vivo gene therapy.

Keywords:
gene deliverypH-sensitiveshielding system

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Area of Science:

  • Biomaterials Science
  • Gene Therapy
  • Nanotechnology

Background:

  • Improving tumor therapeutic efficiency and reducing side effects are critical in gene therapy.
  • Current gene delivery systems face challenges with surface charge and targeted delivery.

Purpose of the Study:

  • To develop and evaluate pH-sensitive ternary complexes for enhanced gene delivery.
  • To investigate the pH-triggered shielding and release capabilities of PEG-b-PLL-g-succinylsulfathiazole (PPSD) coated DNA/PEI polyplexes.

Main Methods:

  • Fabrication of ternary complexes using electrostatic interaction between PPSD and DNA/PEI polyplexes.
  • Assessment of gene transfection efficiency and cell uptake at different pH values (7.4 and 6.0).
  • Evaluation of PPSD's pH-triggered shielding/deshielding properties.

Main Results:

  • PPSD efficiently shielded the surface charge of DNA/PEI polyplexes.
  • Ternary complexes exhibited lower transfection efficiency at pH 7.4 but recovered at pH 6.0 due to pH-triggered DNA/PEI release.
  • Cell uptake results correlated with transfection efficiency trends at varying pH levels.

Conclusions:

  • PPSD demonstrates effective pH-triggered shielding and release of DNA/PEI polyplexes.
  • The developed ternary complexes show potential for targeted in vivo gene delivery.
  • PPSD is a promising shielding system for optimizing gene therapy efficacy and safety.