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An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

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Autotaxin is a novel target of microRNA-101-3p

Yuqin Wang1, Lin Lyu1, Xiaotian Zhang1

  • 1The Key Laboratory of Cell Proliferation and Regulation Biology Ministry of Education Institute of Cell Biology College of Life Sciences Beijing Normal University China.

FEBS Open Bio
|April 16, 2019
PubMed

Insights

MicroRNA-101-3p (miR-101-3p) suppresses tumor growth by downregulating autotaxin (ATX) expression. This study identifies ATX as a novel target of miR-101-3p, revealing a new mechanism in cancer progression.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Autotaxin (ATX) is a key enzyme in the ATX-lysophosphatidic acid (LPA) axis, influencing tumorigenesis.
  • MicroRNA-101-3p (miR-101-3p) is recognized as a tumor suppressor microRNA.

Purpose of the Study:

  • To investigate the regulatory role of miR-101-3p on ATX expression.
  • To elucidate the functional consequences of the miR-101-3p/ATX interaction in cancer cells.

Main Methods:

  • Posttranscriptional regulation analysis
  • Luciferase reporter assays to confirm direct targeting of ATX mRNA by miR-101-3p
  • Cancer cell line experiments (U87, HCT116) to assess ATX and miR-101-3p levels, cell migration, invasion, and proliferation.

Main Results:

  • miR-101-3p directly targets the 3'UTR of ATX mRNA, leading to its downregulation.
  • An inverse correlation between ATX and miR-101-3p expression was observed in cancer cells.
  • miR-101-3p-mediated suppression of migration, invasion, and proliferation was partially reversed by LPA addition.

Conclusions:

  • ATX is identified as a novel direct target of miR-101-3p.
  • The miR-101-3p/ATX axis plays a significant role in regulating cancer cell behaviors.
  • This finding provides new insights into the molecular mechanisms of tumor suppression by miR-101-3p.
Keywords:
autotaxincancer cellinvasionmiR‐101‐3pmigrationproliferation

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