Related Experiment Video
Updated: Jan 26, 2026

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Published on: October 16, 2017
Structural characterization and immunostimulatory activity of a novel polysaccharide isolated with subcritical water
Jixian Zhang1, Meng Chen1, Chaoting Wen1
1School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Abstract:
In the present study, we obtained polysaccharides from Sagittaria sagittifolia L. (SSP) with subcritical water extraction (SWE). Two water-soluble polysaccharides (SSP-W1 and SSP-S1) from the acquired SSP were isolated with DEAE-52 and Sephadex G-100. Besides, the structural characteristics and immunostimulatory activity were also investigated. The results showed that both SSP-W1 and SSP-S1 were homogeneous polysaccharides and the molecular weight was 62.03 KDa and 15.2 KDa, respectively. In addition, both SSP-W1 and SSP-S1 are heteropolysaccharides. Moreover, FT-IR analysis showed that SSP-W1 was α-pyranose polysaccharide, while SSP-S1 was a typical β-pyranose polysaccharide. Congo red staining showed that there was no triple helix structure in both SSP-W1 and SSP-S1. Furthermore, both SSP-W1 and SSP-S1 could promote the proliferation, production of NO, and secretion of TNF-α and IL-10 of macrophages RAW 264.7, significantly. Therefore, the polysaccharides extracted from Sagittaria sagittifolia L. with SWE have the potential to be used as immunoreactive agent in medicine and functional foods.
Related Concept Videos
Biosynthesis of Polysaccharides
Cellulose and Pectic Polysaccharides
As a cell matures, its cell wall specializes according to its type. For example, the...
Water and Mineral Acquisition
Structure-Activity Relationships and Drug Design
SAR studies the intricate relationship between a drug's chemical structure and biological activity. It focuses on understanding how modifications to a drug's structure can influence...
States of Water
Water freezes when the intermolecular forces are greater than the kinetic energy. Unlike most other substances, water is less dense in its solid state than in its liquid state. This is because each water molecule can form...
Cholinergic Antagonists: Chemistry and Structure-Activity Relationship

