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Related Concept Videos

Data Collection II01:29

Data Collection II

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The nursing history captures and records the patient's health status, so that a care plan evolves to meet the patient's individual needs. The nursing health history is a part of the initial assessment. A comprehensive history covers all health dimensions and plays a significant role in the assessment process. A comprehensive history includes the patient's biographical information, reasons for seeking health care, expectations, present and past health history, medications, and...
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Data Collection I01:30

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Data collection gathers information needed to make accurate judgments about a patient's present condition. During a health history interview, subjective data is collected from the patient, their caregivers, or family members, and objective data is collected through observations and physical assessment. Patients are the primary source of subjective data. Thus information gathered from patients through interviews, observations, and physical examination is primary data. Secondary sources of...
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Data Collection by Experiments01:13

Data Collection by Experiments

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Data collection is a systematic method of obtaining, observing, measuring, and analyzing accurate information. An experimental study is a standard method of data collection that involves the manipulation of the samples by applying some form of treatment prior to data collection. It refers to manipulating one variable to determine its changes on another variable. The sample subjected to treatment is known as “experimental units.”
An example of the experimental method is a public...
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Data Collection by Survey01:07

Data Collection by Survey

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The systematic method of obtaining and analyzing accurate information of a population is called data collection. A survey is a standard method of data collection that involves collecting information from a target human population about their experience, opinion, or knowledge of a product, service, or process. The responses are recorded and interpreted. The most common survey examples are written questionnaires, face-to-face or telephonic conversations, focus groups, and electronic (e-mail or...
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Data Collection III01:05

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The physical assessment examines the patient for objective data that defines the patient's condition, and aids in formulating the nursing care plan. The purpose of physical assessment is a health status appraisal, which includes identifying health problems, and establishing a database for nursing intervention.
The principles to begin the physical assessment include conducting a comprehensive or problem-related history in a quiet, well-lit room, emphasizing privacy and comfort for the...
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Data Collection by Observations01:08

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Data collection refers to a systematic way of obtaining, observing, measuring, and analyzing accurate information. Observational studies are one of the most widely used methods of data collection. It involves collecting data by observing the behavior and physical characteristics of a sample without making any modifications to the sample.
An astronomer viewing the motion and brightness of stars in the sky and recording the data is an example of observational data collection. A botanist recording...
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Related Experiment Video

Updated: Jan 26, 2026

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
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MicroED data collection with SerialEM.

M Jason de la Cruz1, Michael W Martynowycz2, Johan Hattne2

  • 1Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA; Structural Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA.

Ultramicroscopy
|April 16, 2019
PubMed
Summary
This summary is machine-generated.

Automated Microcrystal Electron Diffraction (MicroED) using SerialEM software enables high-throughput data collection. This method streamlines the process, allowing over 300 datasets to be collected overnight for large-scale structural analyses.

Keywords:
Electron diffractionMicroEDMicrocrystalSerialEMTransmission electron microscopycryoEM

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Area of Science:

  • Structural Biology
  • Biophysics
  • Materials Science

Background:

  • Microcrystal Electron Diffraction (MicroED) is a powerful cryo-electron microscopy technique for determining molecular structures.
  • Previous MicroED studies relied on manual control of transmission electron microscopes (TEM) and detectors, limiting throughput.
  • Automation is crucial for advancing MicroED applications in large-scale structural biology and materials science.

Purpose of the Study:

  • To develop and present a procedure for automating MicroED data collection.
  • To enable high-throughput structural analysis using MicroED.
  • To facilitate automated data acquisition for protein, peptide, and small molecule structures.

Main Methods:

  • Development of a script for the open-source software SerialEM to automate MicroED.
  • Coordination of stage rotation, microscope operation, and camera functions within SerialEM.
  • Implementation of automated continuous-rotation MicroED data collection.

Main Results:

  • Successful automation of MicroED data collection using SerialEM.
  • Demonstration of high-throughput data acquisition, with over 300 datasets collected overnight.
  • The automated method is adaptable to various crystal and substrate geometries.

Conclusions:

  • Automated continuous-rotation MicroED using SerialEM significantly enhances data collection efficiency.
  • This automated approach facilitates high-throughput MicroED for large-scale structural studies.
  • The developed procedure is a key advancement for structural biology and materials science research.