Related Experiment Video
Updated: Jan 26, 2026

Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning
Published on: November 19, 2018
Quantitative Cytologic Descriptors to Differentiate CLL, Sézary, Granular, and Villous Lymphocytes Through Image
Laura Puigví1,2, Anna Merino1, Santiago Alférez2
1Biomedical Diagnostic Centre, Hospital Clinic of Barcelona, University of Barcelona, Barcelona, Spain.
Objectives:
We aimed to find descriptors to identify chronic lymphocytic leukemia (CLL), Sézary, granular, and villous lymphocytes among normal and abnormal lymphocytes in peripheral blood.
Methods:
Image analysis was applied to 768 images from 15 different types of lymphoid cells and monocytes to determine four discriminant descriptors. For each descriptor, numerical scales were obtained using 627 images from 79 patients. An assessment of the four descriptors was performed using smears from 209 new patients.
Results:
Cyan correlation of the nucleus identified clumped chromatin, and standard deviation of the granulometric curve of the cyan of the nucleus was specific for cerebriform chromatin. Skewness of the histogram of the u component of the cytoplasm identified cytoplasmic granulation. Hairiness showed specificity for cytoplasmic villi. In the assessment, 96% of the smears were correctly classified.
Conclusions:
The quantitative descriptors obtained through image analysis may contribute to the morphologic identification of the abnormal lymphoid cells considered in this article.
Related Concept Videos
Quantitative Analysis
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the...
Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
Cellular Differentiation
A zygote is a...
Differential Leveling
Implicit Differentiation
Logarithmic Differentiation

