Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ion Channels01:19

Ion Channels

91.3K
The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow...
91.3K
Non-gated Ion Channels01:24

Non-gated Ion Channels

8.1K
Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism....
8.1K
G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

5.7K
GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory...
5.7K
Ligand-gated Ion Channels01:19

Ligand-gated Ion Channels

14.1K
Ligand-gated ion channels are transmembrane proteins with a channel for ions to pass through and a binding site for a ligand. The channel opens only when a ligand attaches to the binding site.
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that...
14.1K
Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

10.6K
Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
10.6K
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

7.6K
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
7.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dose escalation in pediatric pelvic Ewing's Sarcoma: Insights from a national treatment planning exercise.

Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)·2025
Same author

[Does radiation therapy for prostate cancer increase the risk of second cancers?]

Cancer radiotherapie : journal de la Societe francaise de radiotherapie oncologique·2024
Same author

Study of curtaining effect reduction methods in Inconel 718 using a plasma focused ion beam.

Journal of microscopy·2024
Same author

Intraoral low-temperature degradation of monolithic zirconia dental prostheses: 5-year results of a prospective clinical study with ex vivo monitoring.

Dental materials : official publication of the Academy of Dental Materials·2023
Same author

Expression of 20 SCPP genes during tooth and bone mineralization in Senegal bichir.

Development genes and evolution·2023
Same author

Pembrolizumab versus cetuximab concurrent with radiotherapy in patients with locally advanced squamous cell carcinoma of head and neck unfit for cisplatin (GORTEC 2015-01 PembroRad): a multicenter, randomized, phase II trial.

Annals of oncology : official journal of the European Society for Medical Oncology·2022

Related Experiment Video

Updated: Jan 26, 2026

Author Spotlight: Exploring Cellular Processes by Modeling Ligands in Cryo-EM Maps
09:30

Author Spotlight: Exploring Cellular Processes by Modeling Ligands in Cryo-EM Maps

Published on: July 19, 2024

2.0K

Angular resolution expected from iCHORD orientation maps through a revisited ion channeling model.

C Langlois1, T Douillard1, S Dubail2

  • 1Université de Lyon, INSA-Lyon, MATEIS CNRS UMR5510, 7 avenue Jean Capelle, 69621 Villeurbanne, France.

Ultramicroscopy
|April 17, 2019
PubMed
Summary

Focused Ion Beam (FIB) microscopy with the ion CHanneling ORientation Determination (iCHORD) method achieves 1° angular resolution for crystalline orientation mapping. This technique offers microstructural characterization comparable to Electron Back Scattered Diffraction without external references.

More Related Videos

Author Spotlight: Exploring the Role of Ion Channels in Cancer: Characterization and Potential Treatment Approaches
06:19

Author Spotlight: Exploring the Role of Ion Channels in Cancer: Characterization and Potential Treatment Approaches

Published on: June 16, 2023

3.8K
Expression and Purification of Mammalian Bestrophin Ion Channels
08:12

Expression and Purification of Mammalian Bestrophin Ion Channels

Published on: August 2, 2018

8.8K

Related Experiment Videos

Last Updated: Jan 26, 2026

Author Spotlight: Exploring Cellular Processes by Modeling Ligands in Cryo-EM Maps
09:30

Author Spotlight: Exploring Cellular Processes by Modeling Ligands in Cryo-EM Maps

Published on: July 19, 2024

2.0K
Author Spotlight: Exploring the Role of Ion Channels in Cancer: Characterization and Potential Treatment Approaches
06:19

Author Spotlight: Exploring the Role of Ion Channels in Cancer: Characterization and Potential Treatment Approaches

Published on: June 16, 2023

3.8K
Expression and Purification of Mammalian Bestrophin Ion Channels
08:12

Expression and Purification of Mammalian Bestrophin Ion Channels

Published on: August 2, 2018

8.8K

Area of Science:

  • Materials Science
  • Solid State Physics
  • Microscopy Techniques

Background:

  • Crystalline orientation mapping is crucial for microstructural characterization.
  • Focused Ion Beam (FIB) microscopy offers advanced imaging capabilities.
  • The ion CHanneling ORientation Determination (iCHORD) method utilizes ion channeling in secondary electron imaging.

Purpose of the Study:

  • To evaluate the angular resolution of the iCHORD method in FIB microscopy.
  • To present a revisited ion channeling model for improved accuracy.
  • To establish a self-referential method for assessing orientation map resolution.

Main Methods:

  • Utilized a revisited ion channeling model to analyze the iCHORD method.
  • Developed a specific procedure to evaluate angular resolution based on orientation errors.
  • Tested the method on a nickel-based sample under standard acquisition conditions.

Main Results:

  • Achieved an angular resolution of 1° for crystalline orientation mapping.
  • Demonstrated the effectiveness of the iCHORD method for microstructural characterization.
  • Validated the self-referential procedure for resolution evaluation.

Conclusions:

  • The iCHORD method in FIB microscopy provides high angular resolution (1°) suitable for microstructural analysis.
  • The developed evaluation procedure is independent of external references.
  • The technique shows potential as an alternative to Electron Back Scattered Diffraction for certain applications.