Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Streptococcus mutans ribosomal preparations: purification and properties.

R L Gregory

    Microbios
    |January 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Evaluation of the antimicrobial potential of fluoride-free mouthwashes against Scardovia wiggsiae.

    European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry·2025
    Same author

    Growth and viability of Streptococcus mutans in sucrose with different concentrations of Stevia rebaudiana Bertoni.

    Clinical oral investigations·2020
    Same author

    Oral Health Effects of Combusted and Smokeless Tobacco Products.

    Advances in dental research·2019
    Same author

    A Case-Control Study to Determine the Prognostic Features of Salivary Epithelial Cells in Periodontitis.

    JDR clinical and translational research·2019
    Same author

    Oral Sciences PhD Program Enrollment, Graduates, and Placement: 1994 to 2016.

    Journal of dental research·2018
    Same author

    Novel bioactive tetracycline-containing electrospun polymer fibers as a potential antibacterial dental implant coating.

    Odontology·2016
    Same journal

    Identification, cloning and expression of sodC from an alkaline phosphatase gene fusion library of Mycobacterium avium subspecies paratuberculosis.

    Microbios·2001
    Same journal

    Distribution of hepatitis C virus serotypes and duration of infection in HCV infected subjects from a geographical area in Southeast Italy.

    Microbios·2001
    Same journal

    Demonstration of genome DNA diversity of strains of urease-positive thermophilic Campylobacter isolated from the natural environment by pulsed-field gel electrophoresis analysis.

    Microbios·2001
    Same journal

    Toxic effects of HgCl2 on the growth and oogonium formation in Oedogonium hatei.

    Microbios·2001
    Same journal

    Growth inhibition of fouling diatoms by antipatharian colonies.

    Microbios·2001
    Same journal

    Inhibition by the essential oils of peppermint and spearmint of the growth of pathogenic bacteria.

    Microbios·2001
    See all related articles

    Streptococcus mutans ribosomes, primarily protein, are immunogenic and can synthesize polypeptides. Protease treatment reduced immunogenicity, while RNase treatment had no effect, indicating proteins are the key antigenic components.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Immunology

    Background:

    • Streptococcus mutans is a key pathogen in dental caries.
    • Ribosomes are essential for protein synthesis and can possess immunogenic properties.

    Purpose of the Study:

    • To characterize the immunogenic properties of Streptococcus mutans ribosomes.
    • To identify the specific components responsible for ribosomal immunogenicity.

    Main Methods:

    • Sucrose density gradient centrifugation to analyze ribosome structure.
    • Electron microscopy for particle size determination.
    • Immunodiffusion, gas chromatography, and Western blot analysis to identify antigenic components.
    • In vitro protein synthesis assays and immunogenicity tests (protease/RNase treatment).

    Related Experiment Videos

    Main Results:

    • Ribosomal preparations from S. mutans were characterized as 70S particles containing RNA and protein.
    • Immunodiffusion revealed reactions with glucosyltransferase and lipoteichoic acid.
    • Gas chromatography detected non-ribosomal carbohydrates (rhamnose, glucose).
    • Ribosomes synthesized polypeptides in vitro, with activity inhibited by anti-ribosomal antibodies.
    • Protease treatment significantly reduced immunogenicity, while RNase treatment had no effect.
    • Western blot identified five immunogenic ribosomal proteins, which were innate components.

    Conclusions:

    • The principal immunogenic moiety of S. mutans ribosomes is protein.
    • Specific ribosomal proteins are responsible for the antigenic properties of S. mutans ribosomes.
    • These findings contribute to understanding the host-pathogen interactions involving S. mutans.