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Updated: Jan 26, 2026

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
Published on: November 16, 2017
Packaging of DNA origami in viral capsids
Idit Kopatz1, Ran Zalk, Yael Levi-Kalisman
1Geza Ad Ltd, Givatayim 53251, Israel. idit.kopatz@geza-ad.com slavakler1@gmail.com.
Abstract:
Here we show the encapsulation of 35 nm diameter, nearly-spherical, DNA origami by self-assembly of SV40-like (simian virus 40) particles. The self-assembly of this new type of nanoparticles is highly reproducible and efficient. The structure of these particles was determined by cryo-EM. The capsid forms a regular SV40 lattice of T = 7d icosahedral symmetry and the structural features of encapsulated DNA origami are fully visible. These particles are a promising biomaterial for use in various medical applications.
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