Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

57.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.6K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.9K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.9K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

659
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
659
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.8K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.8K
Detection of Gross Error: The Q Test01:00

Detection of Gross Error: The Q Test

6.9K
When one or more data points appear far from the rest of the data, there is a need to determine whether they are outliers and whether they should be eliminated from the data set to ensure an accurate representation of the measured value. In many cases, outliers arise from gross errors (or human errors) and do not accurately reflect the underlying phenomenon. In some cases, however, these apparent outliers reflect true phenomenological differences. In these cases, we can use statistical methods...
6.9K
Detection of Black Holes01:10

Detection of Black Holes

2.5K
Although black holes were theoretically postulated in the 1920s, they remained outside the domain of observational astronomy until the 1970s.
Their closest cousins are neutron stars, which are composed almost entirely of neutrons packed against each other, making them extremely dense. A neutron star has the same mass as the Sun but its diameter is only a few kilometers. Therefore, the escape velocity from their surface is close to the speed of light.
Not until the 1960s, when the first neutron...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metal-Organic Framework as a Bioorthogonal Catalyst for Gene Editing.

Journal of the American Chemical Society·2026
Same author

Solvent-Modulated Orthogonal Release from Covalent Organic Frameworks Enables Sequential Multiomics Enrichment.

Journal of the American Chemical Society·2026
Same author

Harnessing a single molecule for dual bioorthogonal regulation of RNA function and m6A methylation.

Nucleic acids research·2026
Same author

Advantages and Challenges of G‑Quadruplexes in Regulating Alternative Splicing.

JACS Au·2026
Same author

Chemical-Assisted Ligation-qPCR for Locus-Specific Detection of RNA <i>N</i><sup>4</sup>-Acetylcytidine and 5-Formylcytidine.

Analytical chemistry·2026
Same author

Targeted detection method for locus-specific m<sup>6</sup>A modifications in low-abundance transcripts based on chemical conversion.

Chemical communications (Cambridge, England)·2026

Related Experiment Video

Updated: Jan 26, 2026

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.3K

N1-Methyladenosine detection with CRISPR-Cas13a/C2c2.

Yi Chen1, Shixi Yang1, Shuang Peng1

  • 1College of Chemistry and Molecular Sciences , Key Laboratory of Biomedical Polymers of Ministry of Education , The Institute for Advanced Studies , Hubei Province Key Laboratory of Allergy and Immunology , Wuhan University , Wuhan , Hubei 430072 , P. R. China . Email: xzhou@whu.edu.cn ; Email: xcweng@whu.edu.cn ; ; Tel: +86-27-68756663.

Chemical Science
|April 19, 2019
PubMed
Summary

Researchers developed a rapid, fluorescence-based method using CRISPR Cas13a to detect N1-methyladenosine (m1A) modifications in RNA. This system shows a significant drop in fluorescence when m1A is present, enabling sensitive m1A quantification.

More Related Videos

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
04:17

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

1.5K
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

2.1K

Related Experiment Videos

Last Updated: Jan 26, 2026

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.3K
DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
04:17

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

1.5K
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

2.1K

Area of Science:

  • Molecular Biology
  • RNA Modifications
  • Biotechnology

Background:

  • N1-methyladenosine (m1A) is an important RNA modification involved in cellular processes like stress response and antibiotic resistance.
  • CRISPR Cas13a is an RNA-targeting enzyme known for its high sensitivity and collateral RNase activity upon target recognition.

Purpose of the Study:

  • To design a rapid, simple, and fluorescence-based detection system for m1A modifications in RNA.
  • To leverage the CRISPR Cas13a system for sensitive detection of m1A-induced mismatches.

Main Methods:

  • Development of a CRISPR Cas13a-based molecular detection platform.
  • Utilizing the collateral activity of Cas13a to detect m1A-induced mismatches in single-stranded RNA (ssRNA).
  • Assessing fluorescence signal changes in the presence of standard RNA (A-ssRNA) versus m1A-modified RNA (m1A-ssRNA).

Main Results:

  • The Cas13a platform exhibited a high fluorescence signal for standard A-ssRNA.
  • A significant decrease (approximately 90%) in fluorescence was observed for m1A-ssRNA, indicating successful detection of the m1A modification.
  • The system demonstrated quantitative capabilities for m1A levels in RNA and was applied to study dynamic m1A demethylation of 28S rRNA using AlkB.

Conclusions:

  • The developed CRISPR Cas13a system provides a sensitive and rapid method for detecting and quantifying m1A RNA modifications.
  • This approach offers a valuable tool for studying the biological roles of m1A and its dynamic regulation, such as in demethylation processes.