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Related Experiment Video

Updated: Jan 26, 2026

BEST: Barcode Enabled Sequencing of Tetrads

BEST: Barcode Enabled Sequencing of Tetrads

Published on: May 1, 2014

BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing.

Daniel Alpern^1,2, Vincent Gardeux^1,2, Julie Russeil¹

¹Institute of Bioengineering, School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.

|April 20, 2019

Summary

This summary is machine-generated.

BRB-seq offers a faster, cheaper, and more accessible method for RNA sequencing (RNA-seq) gene expression analysis. This technique enables genome-wide transcriptomic data generation at a cost comparable to traditional gene profiling methods.

Keywords:

Barcoding Gene expression RNA-seq Transcriptomics qPCR

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RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

Published on: February 13, 2013

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Last Updated: Jan 26, 2026

BEST: Barcode Enabled Sequencing of Tetrads

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Published on: May 1, 2014

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Published on: November 7, 2025

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

Published on: February 13, 2013

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

RNA sequencing (RNA-seq) is crucial for gene expression analysis but remains costly and time-consuming.
Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is a standard but limited method for gene expression analysis.

Purpose of the Study:

To introduce BRB-seq, a novel, cost-effective, and efficient RNA sequencing library preparation method.
To enable high-throughput gene expression analysis for a large number of samples.

Main Methods:

BRB-seq utilizes early multiplexing for 3' cDNA library preparation.
The method requires minimal hands-on time (2 hours) for processing multiple samples simultaneously.

Main Results:

BRB-seq demonstrates comparable performance to the standard TruSeq approach.
BRB-seq exhibits enhanced tolerance for low-quality RNA samples.
BRB-seq is up to 25 times more cost-effective than existing methods.

Conclusions:

BRB-seq significantly reduces the cost and labor associated with RNA-seq.
This method has the potential to democratize genome-wide transcriptomic analysis in basic research labs.