Related Experiment Video
Updated: Jan 26, 2026

LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics
Published on: April 9, 2017
Effect of nonenzymatic deamidation on the structure stability of Camelus dromedarius α-lactalbumin
Saliha Si Ahmed Zennia1, Abderrahmane Mati1, Christophe Charron2
1Université Mouloud Mammeri, Laboratoire de Recherche de Biochimie Analytique et Biotechnologies (LABAB), Tizi Ouzou, Algeria.
Abstract:
Camelid α-lactalbumin is the only known protein that can undergo nonenzymatic deamidation on two Asn residues. This leads to the generation of a mixture of unusual isoAsp and d-Asp residues that may impact health. The effect of deamidation on camel α-lactalbumin instability was investigated. Circular dichroism showed that the altered protein acquired secondary structure resulting in an increase in α-helix content. In good agreement, the 3D structure of camel α-lactalbumin determined by X-ray crystallography, displayed a short additional α-helix probably induced by deamidation, compared to the human and bovine counterparts. This α-helix was located in the C-terminal region and included residues 101-106. Differential scanning calorimetry together with the susceptibility to thermolysin showed that the deamidation process reinforced the structural stability of the α-lactalbumin at high temperature and its resistance toward proteolysis.
Related Concept Videos
Stability of structures
Protein and Protein Structure
A protein's shape is critical to its function. For example, an enzyme...
RNA Stability
Nuclear Stability
To hold positively charged protons together...
Stability
The stability of an LTI system is determined by the roots of its characteristic equation, known as poles. A system is stable if it produces a bounded...
Pole and System Stability
Simple poles are unique roots of the denominator polynomial. Each simple pole corresponds to a distinct solution to the system's characteristic equation, typically resulting in exponential decay terms in the system's...

