Environmental DNA Shaping a New Era of Ecotoxicological Research

Aquatic ecosystems, such as rivers and lakes, are exposed to multiple stressors from anthropogenic activity and changes in climate, which have resulted in a general decrease in biodiversity, alteration of community structures, and can ultimately result in reduction of resources provided by natural ecosystems. Adverse outcomes caused by pollutants to ecosystems are determined not only by toxic properties but also ecological contexts of ecosystems, including indigenous biodiversity and community composition. It is therefore important to identify key factors, such as diversity of species and traits that determine the vulnerability of structures and functions of ecosystems in response to toxic substances. Detection and quantification of biodiversity and its activities using environmental DNA (eDNA) is arguably one of the most important technical advances in ecology in recent years. A huge opportunity has appeared to allow more relevant approaches for assessments of risks posed to ecosystems by toxic substances. eDNA approaches provide effective and efficient tools to evaluate the effects of chemical pollutants on (1) the occurrences and population of wildlife, (2) communities, and (3) the function of ecosystem in the field. Here a conceptual framework of adverse outcome pathways to relate molecular initiating events to apical ecosystem-level responses is proposed to connecting laboratory-based prediction to observations under field conditions. Particularly, future research opportunities on effects on biodiversity, community structure, and ecosystem function by toxic substances will be discussed.