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Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
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A CRACker of an adaptor connects dynein-mediated transport to calcium signaling

Mahak Sharma1, Devashish Dwivedi2

  • 1Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Punjab, India msharma@iisermohali.ac.in.

The Journal of Cell Biology
|April 24, 2019
PubMed

Insights

Two GTPases act as direct dynein adaptors, regulating retrograde transport during endocytosis and responding to calcium signals.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Protein Dynamics

Background:

  • Dynein-dynactin complexes mediate retrograde transport.
  • Adaptor proteins bind cargo receptors like Rab GTP-binding (G) proteins to regulate dynein function.
  • The specific adaptors and their regulation during endocytosis remain incompletely understood.

Purpose of the Study:

  • To identify novel GTPases that function as dynein adaptors.
  • To investigate the role of these GTPases in endocytosis and retrograde transport.
  • To determine the regulatory mechanisms, including calcium dependence, of these novel adaptors.

Main Methods:

  • Biochemical assays to assess GTPase binding to dynein-dynactin.
  • Cellular imaging to track cargo transport during endocytosis.
  • Genetic manipulation to study the function of identified GTPases.

Main Results:

  • Two specific GTPases were identified as direct dynein adaptors.
  • These GTPases facilitate retrograde transport during endocytosis.
  • The activity of these GTPases is regulated by calcium ions.

Conclusions:

  • The identified GTPases represent a novel class of direct dynein adaptors.
  • Calcium-regulated GTPases play a crucial role in endocytic retrograde transport.
  • This finding expands our understanding of the molecular machinery governing intracellular transport.

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