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[Nisin biosynthesis during the joint cultivation of Streptococcus lactis strain MGU and yeasts]
Abstract:
In simultaneous inoculation of the medium for nisin biosynthesis with Streptococcus lactis (strain MSU) and yeasts such as Rhodotorula colostri, Zigowilliopsis californicus, Hansenuia anomala, Saccharomyces ludvigii, Kluyveromyces lactis and Endomyces magnusi the quantitative ratio of the inoculates in the mixed cultures is not in principle important for the antibiotic synthesis by the Streptococcus. When 6-, 12-, 18- and 24-hour inoculates of the yeasts were added simultaneously to 3-, 6-, 9-, 12- and 18-hour cultures of S. lactis biosynthesis of nisin was at the control level. The fractional composition of the fermentation broth of the above yeast species had no significant effect on biosynthesis of nisin.
Insights
The ratio of Streptococcus lactis and yeast inoculates does not impact nisin antibiotic synthesis. Yeast age also has no significant effect on nisin production during fermentation.
Area of Science:
- Microbiology
- Biotechnology
- Food Science
Background:
- Nisin is a bacteriocin produced by Streptococcus lactis with significant antimicrobial properties.
- Understanding factors influencing nisin biosynthesis is crucial for optimizing its production and application.
- The potential synergistic or antagonistic effects of co-culturing S. lactis with yeasts are not fully understood.
Purpose of the Study:
- To investigate the impact of the quantitative ratio of inoculates in mixed cultures of Streptococcus lactis and various yeast species on nisin biosynthesis.
- To determine if the age of yeast inoculates affects nisin production when added to S. lactis cultures.
- To assess the influence of the fermentation broth's fractional composition from different yeast species on nisin biosynthesis.
Main Methods:
- Simultaneous inoculation of S. lactis (strain MSU) with multiple yeast species (Rhodotorula colostri, Zigowilliopsis californicus, Hansenuia anomala, Saccharomyces ludvigii, Kluyveromyces lactis, Endomyces magnusi) in a common medium.
- Varying the quantitative ratios of S. lactis and yeast inoculates in mixed cultures.
- Introducing yeast inoculates of different ages (6, 12, 18, 24 hours) to S. lactis cultures of varying ages (3, 6, 9, 12, 18 hours).
- Analyzing nisin biosynthesis levels under different co-culture conditions and evaluating the effect of yeast fermentation broth composition.
Main Results:
- The quantitative ratio of S. lactis and yeast inoculates in mixed cultures did not significantly affect nisin antibiotic synthesis.
- The age of yeast inoculates (6, 12, 18, 24 hours) had no significant impact on nisin biosynthesis when added to S. lactis cultures of various ages (3-18 hours).
- The fractional composition of the fermentation broth from the tested yeast species did not significantly influence nisin biosynthesis by S. lactis.
Conclusions:
- Nisin biosynthesis by Streptococcus lactis is robust and not critically dependent on the precise inoculum ratios or ages of co-cultured yeasts.
- The presence of various yeast species, under the tested conditions, does not inhibit or significantly enhance nisin production.
- These findings suggest flexibility in optimizing fermentation processes involving S. lactis for nisin production without strict control over yeast co-culture parameters.