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Updated: Jan 25, 2026

Evaluation of Extracellular Vesicle Function During Malaria Infection
Published on: February 14, 2018
Functional Characteristics of Extracellular Vesicles Produced by Pseudomonas fragi ATCC 4973
Robert M Myhara1, Brent J Skura1, Thakor Patel2
1Department of Food Science, University of British Columbia, 6650 N.W. Marine Dr., Vancouver, BC, Canada V6T 1W5.
Abstract:
Extracellular vesicles, "blebs," were isolated from Pseudomas fragi ATCC 4973 grown in liquid culture. Vesicles were isolated by ammonium sulfate precipitation, centrifugation, and dialysis of the supernatant of an 82 h culture of P. fragi in trypticase-soy broth. The vesicles displayed considerable proteolytic activity. The proteinase was similar to that found in a free form in the supernatant. The vesicles were not associated with bacteriocinogenicity. The vesicles may act in the physiological distribution of extracellular proteinases.
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