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Production of Avid Rabbit Antibodies Against Staphylococcal Enterotoxins A and B
Celine Morissette1,2, Jacques Goulet2, Gilles Lamoureux1
1Centre de Recherche en Immunologie, Institut Armand-Frappier, 531 boul, des Prairies, Laval (Québec) H7V 1B7.
Abstract:
The production of avid rabbit antibodies against staphylococcal enterotoxins A (SEA) and B (SEB) is described. The toxic effect of the SEA, measured by the weight loss of immunized rabbits, was greatly minimized by injecting the SEA-anti-SEA serum complex. The anti-SEA and anti-SEB antibody titers were estimated by a rapid ELISA on dipsticks (RD-ELISA) and allowed the selection of avid antibodies capable of reacting within 5 min with immobilized SEA or SEB. Such avid antibodies could be used to develop efficient screening tests for SE in food.
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