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Updated: Jan 25, 2026

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice
Published on: July 25, 2017
Use of Conductance to Detect Bacteriocin Activity
Giorgio Giraffa1, Erasmo Neviani1, Adelia Veneroni1
1Istituto Sperimentale Lattiero Caseario - 20075 Lodi (Italy).
Abstract:
The inhibitory activity of a bacteriocin produced by Lactobacillus delbrueckii subsp. lactis G4 (Bac+) in milk was investigated by using conductivity measurements. The bacteriocin showed an inhibitory action toward some strains belonging to L. delbrueckii subsp. bulgaricus species. A delay in detection time (δDT) of two milk cultures sensitive to bacteriocin, grown in the presence of preformed bacteriocin, was observed. An inactivation as well as a modified growth rate of the sensitive cultures due to bacteriocin activity might explain the δDT, as indicated by longer generation time (tg). Cells showed the highest sensitivity to bacteriocin during the log phase of growth that corresponded to the beginning of the acceleration of the conductance curve (DT).
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