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Updated: Jan 25, 2026

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation
Published on: May 20, 2020
Modified 1-2 Test™ System as a Rapid Screening Method for Detection of Salmonella in Foods and Feeds
John J Oggel1, Dev C Nundy1, Chesley J Randall1
1Laboratory Services Division, Food Production & Inspection Branch, Agriculture Canada, Ottawa, Ontario K1A 0C6.
Abstract:
Modifications made to the procedure for the 1-2 Test™ System (BioControl Systems, Inc., 19805 North Creek Parkway, Bothell, WA 98011) resulted in reducing the time required for analysis by 24 h, while maintaining a high level of agreement with the standard culture procedure. Of 283 samples tested, 73 (25.8%) were found to be naturally contaminated with Salmonella by all analytical conditions combined. The standard culture procedure detected 73 of the contaminated samples, whereas the 1-2 Test™ system, as utilized in this study, found 70 samples to be positive for Salmonella . The modified 1-2 Test™ System procedure yielded a presumptive positive result after 48 h, compared to the 96 h required with the standard culture method, and 72 h with the 1-2 Test™ System as previously evaluated in this laboratory (8).
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