Related Experiment Video
Updated: Jan 24, 2026

Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry
Published on: August 16, 2016
Reliability of the Modified Semi-Solid Rappaport-Vassiliadis Agar and the Modified 1-2 Test™ System for Detection of
John J Oggel1, Dev C Nundy1, P Ann Zebchuk1
1Laboratory Services Division, Food Production and Inspection Branch, Agriculture and Agri-Food Canada, Ottawa, Ontario, K1A 0C6.
Abstract:
The sensitivity of two screening methods for the rapid detection of Salmonella in naturally contaminated poultry feeds was compared to a standard cultural procedure. Of the 1,197 samples of poultry feeds and poultry feed supplements tested, 104 (8.7%) were found to be contaminated with Salmonella by all methods combined. Data from the modified semi-solid Rappaport-Vassiliadis agar (MSRV)/standard cultural procedure comparison showed equivalence of these two methods; 69 of 700 samples analyzed were positive for Salmonella by both methods combined. The false negative rate for the MSRV was 1.0% and for the standard cultural method 1.14%. Comparing the modified 1-2 Test™ and the standard cultural method, the false negative rate was equal at 0.85%, where 85 of 1,061 samples were Salmonella positive by both methods combined. Analysis of 645 samples (56 Salmonella positive) by the three test procedures yielded false negative rates of 1.24% (standard cultural), 0.93% (MSRV) and 1.09% (Modified 1-2 Tests™). The false positive rates for the three methods were: standard cultural - 0.08%; MSRV - 0.86%; modified 1-2 Test™ - 1.04%. The agreement between the three methods of analysis was satisfactory overall, indicating that both the MSRV and the Modified 1-2 Test™ procedures are reliable methods for Salmonella analysis of poultry feeds and poultry feed supplements.
More Related Videos
08:19Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products
Published on: January 31, 2025
09:10Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
Published on: October 18, 2010
Related Concept Videos
Modified Boxplots
However, the box plot does not tell the reader about outliers - values that lie far from the center of the data. We can modify the standard box and whisker plot to identify the outliers and visualize the actual spread of the data in a sample.
Initially, we calculate the adjusted...
Antiasthma Drugs: Leukotriene Modifiers
Leukotriene modifiers work through two distinct mechanisms:
Predicting Products: SN1 vs. SN2
With increased substitution on the alkyl halide,...
Reliability and Validity
Metallic Solids
All metallic solids exhibit high thermal and electrical conductivity, metallic luster, and malleability....
Structures of Solids