Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

9.2K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
9.2K
COP Coated Vesicles00:59

COP Coated Vesicles

17.4K
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
17.4K
Coat Assembly and GTPases01:33

Coat Assembly and GTPases

4.3K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
4.3K
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

4.0K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
4.0K
Sensitivity, Specificity, and Predicted Value01:13

Sensitivity, Specificity, and Predicted Value

1.2K
In healthcare diagnostics, laboratory tests play a crucial role in identifying and diagnosing a wide range of medical conditions. However, interpreting test results is not always straightforward. An abnormal test result does not always confirm the presence of a disease, just as a normal result does not guarantee its absence. To assess the reliability of these diagnostic tools, healthcare practitioners rely on two key statistical indicators: sensitivity and specificity.
Sensitivity is the...
1.2K
Sputum Studies II: Culture and Sensitivity01:20

Sputum Studies II: Culture and Sensitivity

1.2K
Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolomic data of melittin-intervened murine cervical cancer cells based on liquid chromatography-mass spectrometry.

Data in brief·2026
Same author

Allergic Sensitization to Common Ragweed and Mugwort Pollen Allergens: A 6-Year Single-Center Retrospective Analysis.

Journal of immunology research·2026
Same author

From discarding to leveraging: quality-aware collaborative learning for robust diabetic retinopathy grading.

Frontiers in medicine·2026
Same author

Unraveling the Design Principles of Crystallographic Orientation for Ultralong, 8500 Cycled Fe-Ion Battery.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

A highly stretchable tri-channel fiber for composite motion decoupling.

Nature communications·2026
Same author

Periodic Molecular-Level Asymmetric Channels for Synergistical Purification of Iodide Wastewater and Osmotic Power Generation.

Journal of the American Chemical Society·2026

Related Experiment Video

Updated: Jan 25, 2026

Biodegradable Magnesium Stent Treatment of Saccular Aneurysms in a Rat Model - Introduction of the Surgical Technique
11:07

Biodegradable Magnesium Stent Treatment of Saccular Aneurysms in a Rat Model - Introduction of the Surgical Technique

Published on: October 1, 2017

11.6K

A pH-sensitive self-healing coating for biodegradable magnesium implants.

Pan Xiong1, JiangLong Yan1, Pei Wang1

  • 1Biomed-X Center, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.

Acta Biomaterialia
|April 29, 2019
PubMed
Summary
This summary is machine-generated.

This study developed a biocompatible self-healing coating for biomedical magnesium alloys using silk fibroin and phosphate. The coating enhances corrosion resistance and promotes bone growth, offering a promising advancement for medical implants.

Keywords:
BiodegradationBiomedical Mg alloyOsteogenic activitySelf-healingSilk fibroin

More Related Videos

Direct and Indirect Culture Methods for Studying Biodegradable Implant Materials In Vitro
14:49

Direct and Indirect Culture Methods for Studying Biodegradable Implant Materials In Vitro

Published on: April 15, 2022

5.6K
Graphene Coatings for Biomedical Implants
13:21

Graphene Coatings for Biomedical Implants

Published on: March 1, 2013

21.7K

Related Experiment Videos

Last Updated: Jan 25, 2026

Biodegradable Magnesium Stent Treatment of Saccular Aneurysms in a Rat Model - Introduction of the Surgical Technique
11:07

Biodegradable Magnesium Stent Treatment of Saccular Aneurysms in a Rat Model - Introduction of the Surgical Technique

Published on: October 1, 2017

11.6K
Direct and Indirect Culture Methods for Studying Biodegradable Implant Materials In Vitro
14:49

Direct and Indirect Culture Methods for Studying Biodegradable Implant Materials In Vitro

Published on: April 15, 2022

5.6K
Graphene Coatings for Biomedical Implants
13:21

Graphene Coatings for Biomedical Implants

Published on: March 1, 2013

21.7K

Area of Science:

  • Biomaterials Science
  • Surface Engineering
  • Biomedical Engineering

Background:

  • Magnesium alloys are promising for biomedical implants but require surface modification to improve biocompatibility and corrosion resistance.
  • Existing self-healing coatings lack the necessary biocompatibility for biomedical applications.
  • Developing functional coatings for magnesium alloys is crucial for enhancing implant performance and longevity.

Purpose of the Study:

  • To fabricate a novel self-healing coating on biomedical Mg-1Ca alloy.
  • To evaluate the coating's self-healing capacity, corrosion inhibition, and biocompatibility.
  • To assess the coating's potential for promoting osteogenic activity.

Main Methods:

  • Compositing silk fibroin and potassium phosphate (K3PO4) to create a self-healing coating.
  • Utilizing scratch testing, scanning vibrating electrode technique (SVET), and electrochemical impedance spectroscopy (EIS) for performance evaluation.
  • Conducting cell culture studies (MC3T3-E1) to assess biocompatibility and osteogenic activity.

Main Results:

  • The composite coating demonstrated pH-sensitive self-healing capabilities, effectively recovering barrier properties.
  • Phosphate ions acted as corrosion inhibitors, while silk fibroin regulated coating structure.
  • The coating exhibited excellent biocompatibility, promoting cell adhesion, proliferation, and differentiation, indicating osteogenic potential.

Conclusions:

  • A novel, biocompatible, self-healing coating was successfully developed for biomedical Mg-1Ca alloy.
  • The coating provides enhanced corrosion resistance and osteogenic activity, crucial for biomedical applications.
  • This research opens new avenues for advanced surface modifications of magnesium alloys in the biomedical field.