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Engineering glucose oxidase for bioelectrochemical applications.
1Centre de Recherche Paul Pascal (CRPP), CNRS UMR 5031, 115 Avenue du Docteur Schweitzer, Pessac 33600, France; University of Bordeaux, CRPP, CNRS UMR 5031, 115 Avenue du Docteur Schweitzer, Pessac 33600, France.
Bioelectrochemistry (Amsterdam, Netherlands)
|April 29, 2019
Summary
Enzyme engineering advances glucose oxidase (GOx) for better electrochemical sensors. This review highlights mutations to improve electron transfer and reduce oxygen sensitivity for next-generation glucose monitoring.
Area of Science:
- Biochemistry
- Electrochemistry
- Biotechnology
Background:
- Glucose oxidase (GOx) is crucial for glucose sensor development, with over 1000 papers published annually since 2012.
- Existing commercial sensors often fail to address GOx limitations, such as oxygen sensitivity and electron transfer competition with redox mediators.
Purpose of the Study:
- This review focuses on the specific reengineering of glucose oxidase for electrochemical applications.
- It highlights recent progress in GOx engineering to overcome limitations for improved glucose sensor performance.
Main Methods:
- Discusses enzyme engineering strategies and specific mutations applied to glucose oxidase.
- Reviews methods for screening engineered GOx variants, emphasizing electrochemical probing.
Main Results:
- Engineered GOx variants show increased electron transfer rates to electrode surfaces.
- Mutations are effective in decreasing the oxygen sensitivity of glucose oxidase.
Conclusions:
- Further development of electrochemical screening methods is essential for advancing glucose sensor technology.
- The goal is to create next-generation glucose sensors that are glucose-specific, oxygen-independent, biocompatible, and stable for at least two weeks.
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