Rapid Dissolution of Amyloid β Fibrils by Silver Nanoplates

Plaques of amyloid beta (Aβ) protein are associated with neurodegenerative diseases, and preventing their formation and dissolution of plaques are essential to the development of therapeutics. In this study, silver triangular nanoplates (AgTNPs) are shown to dissolve mature Aβ fibrils because of their plasmonic photothermal property. Mature Aβ fibrils treated with AgTNPs under near-infrared (NIR)-illuminated conditions are dissolved in less than 1 h, while an equal concentration of silver spherical nanoparticles took about 70 h. The concentration of the fibrils decreased from 10 to 0.3 μM upon treating the amyloid fibrils with AgTNPs under NIR. AgTNPs are also shown to prevent the formation of Aβ fibrils by selective binding to the positively charged amyloidogenic sequence of the Aβ monomer. The kinetics of inhibition by AgTNPs follows the predictions of the detailed kinetic model (Ramesh et al., Langmuir 2018, 34, 4004-4012). The kinetics of dissolution and inhibition are characterized by Congo red/ThT assay, transmission electronic microscopy, atomic force microscopy, and attenuated total reflectance Fourier transform-infrared spectroscopy. Cell viability studies on SH-SY5Y and BE-(2)-C cells using 3-[4,5-dimethy-lthi-azol-2-yl]-2,5-diphenyl-tetrazdium bromide and lactate dehydrogenase assay show that the viability of the cells increased from 33 to 70% on treating the cells with AgTNP-incubated Aβ fibrils compared to the mature Aβ fibrils. The study provides new insights to design plasmonic nanoparticle-based therapeutics to cure neurodegenerative diseases.