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Labeled quantitative mass spectrometry to study the host response during aspergillosis in the common bottlenose

Guillaume Desoubeaux1, Maria Del Carmen Piqueras2, Carolina Le-Bert3

  • 1University of Miami, Division of Comparative Pathology, Department of Pathology & Laboratory Medicine, Miller School of Medicine, Miami, FL, 33136, USA; CHU de Tours, Service de Parasitologie, Mycologie, Médecine tropicale, 37044, Tours, France; Université de Tours, CEPR - INSERM U1100 / Équipe 3, Faculté de Médecine, 37032, Tours, France.

Veterinary Microbiology
|April 30, 2019
PubMed

Insights

Proteomics identified key protein changes in dolphins with Aspergillosis, revealing insights into the fungal infection

Area of Science:

  • Marine Mammal Health
  • Mycology
  • Proteomics

Background:

  • Aspergillosis, a fungal infection, poses challenges in animal and human medicine.
  • Understanding Aspergillosis pathophysiology in animals is crucial for new diagnostic and therapeutic strategies.
  • Proteomics offers a method to study protein alterations during disease states.

Purpose of the Study:

  • To investigate protein expression changes in bottlenose dolphins with Aspergillosis.
  • To identify potential biomarkers for Aspergillosis diagnosis and treatment targets.
  • To evaluate the utility of isobaric tagging for relative and absolute quantitation (iTRAQ) in cetacean blood samples.

Main Methods:

  • Blood samples from Aspergillus-diseased, healthy, convalescent, and non-Aspergillus inflamed dolphins were analyzed.
  • Mass spectrometry-based iTRAQ analysis was employed for protein identification and quantification.
  • Statistical analysis identified significantly over- and underrepresented proteins.

Main Results:

  • Sixty-six proteins were significantly overrepresented, and 40 were underrepresented in diseased dolphins compared to those with other inflammatory conditions.
  • Many identified proteins are involved in adaptive immune response, including MHC proteins and NADPH-ubiquinone oxido-reductases.
  • Proteomic findings were largely consistent across comparisons with clinically-normal and convalescent dolphins.

Conclusions:

  • iTRAQ is a valuable proteomic tool for ex vivo studies on animal pathophysiology.
  • This study provides a foundation for validating new diagnostic markers for Aspergillosis in dolphins.
  • Further research can build upon these proteomic findings to improve Aspergillosis management.
Keywords:
AspergillosisDolphinInterleukinOrbitrapPrepronociceptinProtamine P1Tursiops truncates

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