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Structural Influence on the Post-Clustering Stability of DNA/AgNCs Fluorescence
Riddhi Nagda1, Pratik Shah2, Chang Seop Lee3
1Department of Systems Biology, College of Life Science and Biotechnology, Institute of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea. riddhinagda@yonsei.ac.kr.
Nanomaterials (Basel, Switzerland)
|May 1, 2019
Summary
DNA-encapsulated Silver Nanoclusters (DNA/AgNCs) exhibit varying fluorescence stability based on DNA structure. Dimer DNA templates yield more stable orange fluorescence, while hairpin templates show rapid decay in red fluorescence due to oxidation.
Area of Science:
- Nanomaterials Science
- Biophysical Chemistry
- Analytical Chemistry
Background:
- DNA-encapsulated Silver Nanoclusters (DNA/AgNCs) are promising for bioimaging and biosensing.
- Understanding the long-term fluorescence stability of DNA/AgNCs is crucial for practical applications.
- The relationship between DNA structure and DNA/AgNCs fluorescence stability requires further investigation.
Purpose of the Study:
- To investigate the correlation between DNA secondary structures and the long-term fluorescence stability of DNA/AgNCs.
- To compare the stability of AgNCs encapsulated by dimer versus hairpin DNA templates.
Main Methods:
- Synthesis of DNA/AgNCs using standardized locking-to-unlocking sensor designs.
- Monitoring of fluorescence and DNA template structures over three weeks.
- Utilized fluorescence spectroscopy and in-gel fluorescent assays.
Main Results:
- AgNCs encapsulated by dimer-structured DNA templates exhibited greater fluorescence stability compared to hairpin structures.
- Orange fluorescence from dimer-encapsulated AgNCs increased over three weeks, while red fluorescence from hairpin-encapsulated AgNCs decreased by over 80% within two days.
- Hairpin-encapsulated AgNCs were more susceptible to oxidation by atmospheric oxygen than dimer-encapsulated AgNCs.
Conclusions:
- Tuning DNA secondary structures is a viable strategy for enhancing the photophysical stability of AgNCs.
- Dimer DNA templates provide superior encapsulation for stable, orange-emissive AgNCs.
- This approach is essential for the pragmatic application of DNA/AgNCs in various fields.
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