Exosomal miR-301 derived from mesenchymal stem cells protects myocardial infarction by inhibiting myocardial

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Pluripotent Stem Cell Derived Cardiac Cells for Myocardial Repair
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Pluripotent Stem Cell Derived Cardiac Cells for Myocardial Repair

Published on: February 3, 2017

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Yong Li1, Rong Yang2, Bingyan Guo2

  • 1Department of Cardiology, The Second Hospital of Hebei Medical University, No. 215, Heping West Road, Shijiazhuang City, Hebei Province, 050000, China; Department of Cardiology, Harrision International Peace Hospital, No. 180, Renmin East Road, Hengshui City, Hebei Province, 053000, China.

Biochemical and Biophysical Research Communications
|May 1, 2019
PubMed
Abstract

Insights

MicroRNA-301 delivered via bone mesenchymal stem cell exosomes protects against myocardial infarction in rats by inhibiting autophagy. This finding offers a potential therapeutic strategy for heart attack recovery.

Area of Science:

  • Cardiovascular Research
  • Stem Cell Biology
  • Molecular Medicine

Background:

  • Myocardial infarction (MI) remains a leading cause of mortality worldwide.
  • Bone mesenchymal stem cells (BMSCs) show therapeutic potential for cardiovascular diseases.
  • Exosomes derived from BMSCs are investigated as cell-free therapeutic agents.

Purpose of the Study:

  • To investigate the protective effects of miR-301 carried in BMSC-secreted exosomes on myocardial infarction in a rat model.
  • To elucidate the mechanism underlying the cardioprotective effects of miR-301 exosomes.

Main Methods:

  • BMSCs were isolated, cultured, and identified. Exosomes were extracted from BMSC cultures.
  • Rat myocardial infarction models were established and treated with PBS, BMSC-exosomes, or miR-301-mimic transfected BMSC-exosomes.
  • Cardiac function, infarct size, gene/protein expression, and autophagy markers were assessed.

Main Results:

  • Rats treated with miR-301-enriched BMSC-exosomes showed improved cardiac function (LVEF, LVFS) and reduced infarct size compared to controls.
  • Increased miR-301 and P62 expression, along with decreased LVESD, LVEDD, LC3-II/LC3-I ratio, and autophagosome numbers were observed in the miR-301 group.
  • These results indicate that miR-301 in BMSC-exosomes modulates myocardial autophagy.

Conclusions:

  • miR-301 delivered by BMSC-derived exosomes exerts a protective effect against myocardial infarction in rats.
  • The cardioprotective mechanism involves the inhibition of myocardial autophagy.
  • This study highlights the therapeutic potential of exosomal miR-301 for treating myocardial infarction.
Keywords:
AutophagyBMSCsExosomesMyocardial infarctionmiR-301

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