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Updated: Jan 25, 2026

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
Published on: August 9, 2022
A ribose modification of Spinach aptamer accelerates lead(ii) cation association in vitro
Jonathan C Savage1, Pushkar Shinde2, Hans Peter Bächinger3
1Department of Biochemistry & Molecular Biology, Oregon Health & Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA. shindeu@ohsu.edu.
Abstract:
Spinach aptamer fluorescence requires formation of a tripartite complex composed of folded RNA, a GFP-like fluorophore, and selective cation coordination. 2'F pyrimidine modified Spinach has retained fluorescence, increased chemical stability, and accelerated cation association via increased G-quadruplex dynamics, thereby reducing readout time and enhancing Spinach utility for aqueous Pb2+ detection.
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