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Updated: Jan 25, 2026

Measuring Granulocyte and Monocyte Phagocytosis and Oxidative Burst Activity in Human Blood
Published on: September 12, 2016
Short Communication: Effect of Seminal Plasma on Functions of Monocytes and Granulocytes
Kevin J Selva1, Jennifer A Juno1, Matthew J Worley1
11 Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Australia.
Abstract:
Most HIV-1 transmissions occur at mucosae and involve exposure to semen. Semen contains immunomodulatory factors, which inhibit anti-HIV-1 natural killer cell and T cell responses. We demonstrate high concentrations (1:2 dilution) of seminal plasma (SP) inhibit monocyte phagocytosis and anti-HIV-1 Fc-dependent functions of both neutrophils and monocytes. In addition, slightly lower SP concentrations (1:2-1:10 dilutions) inhibit granulocyte phagocytosis and oxidative burst of both monocytes and granulocytes. These observations may have implications for HIV-1 infectivity after mucosal exposure.
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