Related Experiment Video
Updated: Jan 25, 2026

Author Spotlight: Elucidating the Dynamics of Mechano-Transduction and Nuclear Agitation in Mouse Oocytes
Published on: January 12, 2024
A microfluidic platform with cell-scale precise temperature control for simultaneous investigation of the osmotic
Zeling Lei1, Dongcheng Xie2, Momoh Karmah Mbogba1
1Department of Electronic Science and Technology, University of Science and Technology of China, Hefei 230027, Anhui, China. zhaog@ustc.edu.cn.
Abstract:
The temperature-dependent oocyte membrane permeability plays a significant role in oocyte cryopreservation, such as optimizing the addition/removal of cryoprotective agents and the rate of cooling/rewarming. However, the systems for studying the temperature dependence of oocyte membrane permeability are either too complicated or unable to achieve wide-range precise temperature control. In addition, these systems cannot achieve the simultaneous observation of multiple oocytes. Here, we report a novel microfluidic platform that combines a precise local temperature heater/detector and a simple global water bath to achieve wide-range accurate temperature control without increasing the difficulty of fabrication, and it also realizes non-interfering, position-controllable and non-missing capture of multiple oocytes for parallel experiments to increase throughput. The permeability coefficients (Lp, Ps) of the mouse oocyte membrane exposed to cryoprotective agents (1.5 M EG and 1.5 M PG) at four temperatures (4, 15, 25 and 37 °C) are consistent with those reported in previous works, which proves the feasibility and practicality of the microfluidic platform in this study.
More Related Videos
11:23A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
Published on: October 6, 2019
11:32A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
Published on: November 23, 2015
Related Concept Videos
Thermometers and Temperature Scales
As many physical properties depend on temperature, the variety of thermometers is...
Osmosis and Osmotic Pressure of Solutions
Physical Methods for Controlling Microbial Growth: Temperature
Uncertainty in Measurement: Accuracy and Precision
pH Scale
Responses to Heat and Cold Stress