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Light enters the eye through the cornea, a transparent, dome-shaped surface covering the surface of the eyeball that helps to direct and focus incoming light. This light is then channeled toward the pupil, an adjustable opening whose size is controlled by the iris. The iris, a pigmented muscle, regulates the amount of light entering the eye by contracting or dilating the pupil, thereby ensuring optimal light levels for clear vision.
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Visualizing Microglia with a Fluorescence Turn-On Ugt1a7c Substrate.

Beomsue Kim1, Masahiro Fukuda2, Jung-Yeol Lee3,1,4

  • 1Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), Singapore, 138667, Singapore.

Angewandte Chemie (International Ed. in English)
|May 1, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed CDr20, a novel fluorogenic chemical probe for visualizing microglia in the brain. This probe targets the Ugt1a7c enzyme, enabling fluorescence in microglia for advanced imaging applications.

Keywords:
biological activityfluorescent probesimaging agentsmicrogliastructure-activity relationships

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biochemistry

Background:

  • Microglia, the brain's immune cells, are crucial for development and disease.
  • Current live microglia imaging is limited, primarily to transgenic models.
  • New methods for microglia detection and imaging are needed.

Purpose of the Study:

  • To develop a novel chemical probe for high-resolution imaging of live microglia.
  • To identify the molecular target of the developed probe.

Main Methods:

  • Structure-activity relationship studies to design the probe CDr20.
  • Genome-scale CRISPR-Cas9 knockout screening to identify the probe's target.
  • In vitro and in vivo imaging experiments.

Main Results:

  • CDr20, a high-performance fluorogenic chemical probe, was successfully developed.
  • UDP-glucuronosyltransferase Ugt1a7c was identified as the target of CDr20.
  • CDr20 enables visualization of microglia through Ugt1a7c-mediated glucuronidation.

Conclusions:

  • CDr20 facilitates non-invasive, cellular-resolution imaging of microglia in vitro and in vivo.
  • The discovery of Ugt1a7c as the target provides mechanistic insight into CDr20's function.
  • This probe represents a significant advancement for studying microglia in health and disease.