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Updated: Jan 25, 2026

Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture
Published on: July 21, 2020
Differential Effects of Nano TiO₂ and CeO₂ on Normal Human Lung Epithelial Cells In Vitro
Sheau-Fung Thai1, Carlton P Jones1, Garret B Nelson1
1National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, 109 TW Alexander Dr., Durham, NC 27711, USA.
Abstract:
Nano-TiO₂ and nano-CeO₂ are among the most widely used engineered nanoparticles (NPs). We investigated a variety of endpoints to assess the toxicity of eight of these NPs to induce potentially adverse health effects in an In Vitro human respiratory epithelial cell model. These endpoints include cytotoxicity, reactive oxygen species (ROS)/reactive nitrogen species (RNS) production, 8-hydroxy-2_-deoxyguanosine (8-oxo-dG), endogenous DNA adducts, Apurinic/apyrimidinic (AP) sites, 4-Hrdoxynonenal (4-HNE) protein adducts, Malondialdehyde (MDA) protein adducts, and genomics analysis on altered signaling pathways. Our results indicated that cytotoxicity assays are relatively insensitive, and we detected changes in other endpoints at concentrations much lower than those inducing cytotoxicity. Among the ROS-related endpoints, 8-oxo-dG is relatively more sensitive than other assays, and nano-TiO₂ induced more 8-oxo-dG formation than nano-CeO₂. Finally, there are many signaling pathways changes at concentrations at which no cytotoxicity was observed. These alterations in signaling pathways correlated well with In Vitro toxicity that was observed at higher concentrations, and with in vivo adverse outcome pathways caused by nano-TiO₂ and nano-CeO₂ in experimental animals.
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