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Updated: Jan 25, 2026

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
Published on: April 21, 2023
Surfactant Assisted Hydroxyapatite Nanoparticles: Drug Loading and In Vitro Leaching Kinetics and Antimicrobial
Varun Prasath Padmanabhan1, Ravichandran Kulandaivelu1, Devendrapandi Santhana Panneer2
1Department of Analytical Chemistry, University of Madras, Guindy Campus Chennai 600025, Tamilnadu, India.
Abstract:
In the present investigation, extremely fine Hydroxyapatite (HAP) powder was prepared through chemical precipitation technique under the influence of a nonionic surfactant (Brij 35-Polyoxyethylene lauryl ether). The samples were sintered at 800 °C to obtain highly pure HAP phase. The functional group present in the HAP powder was confirmed by using Fourier transform infra-red (FTIR) spectroscopy. The change in Phase transformation, the crystallite size and the percentage of crystallinity of the synthesized sample were studied by X-ray diffractometer technique. The Field Emission Scanning Electron Microscope (FESEM) and High-resolution transmission electron microscopy (HRTEM) were used for the structural analysis of porous rod-shaped HAP crystal. Further, drug loading and In Vitro leaching kinetics were conducted for antibiotics-ciproflaxin (CPF). Antimicrobial activity against S. aureus (Gram-positive bacterium) and E. coli (Gram-negative bacterium) was executed for pure HAP as well as for drug loaded HAP.
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